Part I: Overview
1. The Factory of Blood Production: Hematopoietic Stem Cells
Jonathan Hoggatt and Louis M. Pelus
Part II: Molecular Modification and Evaluation
2. Detection of DNA Damage in Hematopoietic Stem Cells
Saipriya Ayyar and Isabel Beerman
3. Evaluating Histone Acetylation in Mouse Hematopoietic Stem and Progenitor Cells Using Chromatin Immunoprecipitation
Liqiong Liu
4. CRISPR Gene Editing of Hematopoietic Stem and Progenitor Cells
Reza Shahbazi, Patricia Lipson, Karthikeya S.V. Gottimukkala, Daniel D. Lane, and Jennifer E. Adair
5. Lentiviral Transduction of Nonhuman Primate Hematopoietic Stem and Progenitor Cells
Chuanfeng Wu, So Gun Hong, Aylin Bonifacino, and Cynthia E. Dunbar
Part III: Cellular Isolation and Characterization
6. Identification of Nonhuman Primate Hematopoietic Stem and Progenitor Cells
Stefan Radtke and Hans-Peter Kiem
7. Isolation and Characterization of Fetal Liver Hematopoietic Stem Cells
Diego A. López and Anna E. Beaudin
8. Utilizing CyTOF to Examine Hematopoietic Stem and Progenitor Phenotype
Safa F. Mohamad and Maegan L. Capitano
9. Hematopoietic Stem Cell Identification Post-Radiation
Andrea M. Patterson, Christie M. Orschell, and Louis M. Pelus
Part IV: The Hematopoietic Niche and Accessory Cells
10. Intravital Microscopy for Hematopoietic Studies
Myriam Haltalli and Cristina Lo Celso
11. Laser Micromachining of Bone as a Tool for Studying Bone Marrow Biology
Christa Haase, Dmitry Richter, and Charles P. Lin
12. MSC and HSPC Co-Culture: Mimicking Ex Vivo Bone Marrow Niche
Pratibha Singh
13. Isolation of Thymus Stromal Cells from Human and Murine Tissue
Karin Gustafsson and David T. Scadden
Part V: Transplantation and In Vivo Models
14. Experimental Models of Mouse and Human Hematopoietic Stem Cell Transplantation
Scott H. Cooper, Maegan L. Capitano, and Hal E. Broxmeyer
15. Hematopoietic Stem and Progenitor Cell Identification and Transplantation in Zebrafish
Ellen Fraint, Peng Lv, Feng Liu, Teresa V. Bowman, and Owen J. Tamplin
16. Establishing a Murine Model of the Hematopoietic Acute Radiation Syndrome
P. Artur Plett, Louis M. Pelus, and Christie M. Orschell
Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.
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Etat : New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. This detailed volume collects updates on the technical advances in hematopoietic stem cell research and incorporates new techniques focused on the molecular/genetic, cellular, and whole organism levels. Exploring methods that apply stress to hematopoiesis, . N° de réf. du vendeur 600113347
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Buch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed volume collects updates on the technical advances in hematopoietic stem cell research and incorporates new techniques focused on the molecular/genetic, cellular, and whole organism levels. Exploring methods that apply stress to hematopoiesis, the book also contains chapters focused on better understanding the role of hematopoietic niches and their cellular components, as well as in vivo models that test and quantitate stem cell function and are key to further development of therapeutic applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and up-to-date, Hematopoietic Stem Cells: Methods and Protocols serves as a valued addition to laboratories focused on understanding hematopoietic stem cell biology and the therapeutic advances that can be derived from it. 296 pp. Englisch. N° de réf. du vendeur 9781071626788
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