1. Cartilage Tissue Engineering: An Introduction
Martin J. Stoddart, Elena Della Bella, and Angela R. Armiento
2. Chondrocyte Isolation and Expansion
Paul Harrison, Timothy Hopkins, Charlotte Hulme, Helen McCarthy, and Karina Wright
3. Isolation of Chondrons from Hyaline Cartilage
Jasmijn V. Korpershoek, Margot Rikkers, and Lucienne A. Vonk
4. Articular Cartilage Chondroprogenitors: Isolation and Directed Differentiation
Ilyas M. Khan, Joshua McKenna, and Yadan Zhang
5. Physioxic Culture of Chondrogenic Cells
Girish Pattappa, Brandon D. Markway, Denitsa Docheva, and Brian Johnstone
6. Isolation and In Vitro Chondrogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stromal Cells
Angela R. Armiento, Yann D. Ladner, Elena Della Bella, and Martin J. Stoddart
7. Adipose-Derived Stromal Cells: Isolation, Expansion, and Differentiation
Justyna M. Buchert, Benedict Lotz, Solvig Diederichs, and Wiltrud Richter
8. Chondrogenic Differentiation of Human Induced Pluripotent Stem Cells
Amanda R. Dicks, Nancy Steward, Farshid Guilak, and Chia-Lung Wu
9. The Dimethylmethylene Blue Assay (DMMB) for the Quantification of Sulphated Glycosaminoglycans
Yann D. Ladner, Mauro Alini, and Angela R. Armiento
10. Extracellular Vesicle Isolation and Characterization for Applications in Cartilage Tissue Engineering and Osteoarthritis Therapy
Alexander Otahal, Andrea De Luna, Ali Mobasheri, and Stefan Nehrer
11. Metabolomic Profiling to Understand Chondrocyte Metabolism
Priyanka P. Brahmachary, Hope D. Welhaven, and Ronald K. June
12. Techniques for Visualization and Quantification of Primary Cilia in Chondrocytes
Huan Meng, Clare L. Thompson, Clarissa Coveney, Angus K. Wann, and Martin M. Knight
13. Co-Culture of Human Articular Chondrocytes Seeded in Polyurethane Scaffolds and Human Mesenchymal Stromal Cells Encapsulated in Alginate Beads
Teresa Z. Brose, Yann D. Ladner, E. Johanna Kubosch, Martin J. Stoddart, and Angela R. Armiento
14. Isolation of Murine Articular Chondrocytes for Single Cell RNA or Bulk RNA Sequencing Analysis
Jillian L. McCool, Nicholas R. Hum, Aimy Sebastian, and Gabriela G. Loots
15. Modulation of MicroRNA Expression during In Vitro Chondrogenesis
Austin Bell-Hensley, Hongjun Zheng, and Audrey McAlinden
16. Purification and Isolation of Proteins from Hyaline Cartilage
Makenna J.
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Etat : New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. This detailed book brings together a collection of methodologies, from the most basic to the more complex, that provides researchers with a platform they can use to embark on a cartilage research career. To aid in the search for novel therapies for cartilag. N° de réf. du vendeur 668436044
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Buch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed book brings together a collection of methodologies, from the most basic to the more complex, that provides researchers with a platform they can use to embark on a cartilage research career. To aid in the search for novel therapies for cartilage regeneration, this volume addresses 3D cartilage models, challenges associated with RNA and protein extraction, imaging, gene transfer, as well as stable differentiation and variations in cell phenotype from different tissue origins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and up-to-date, Cartilage Tissue Engineering serves as an ideal guide for researchers working to advance the vital study of cartilage biology and repair. 412 pp. Englisch. N° de réf. du vendeur 9781071628386
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Buch. Etat : Neu. This item is printed on demand - Print on Demand Titel. Neuware -This detailed book brings together a collection of methodologies, from the most basic to the more complex, that provides researchers with a platform they can use to embark on a cartilage research career. To aid in the search for novel therapies for cartilage regeneration, this volume addresses 3D cartilage models, challenges associated with RNA and protein extraction, imaging, gene transfer, as well as stable differentiation and variations in cell phenotype from different tissue origins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Libri GmbH, Europaallee 1, 36244 Bad Hersfeld 412 pp. Englisch. N° de réf. du vendeur 9781071628386
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