The Ubiquitin Code - Couverture souple

Couverture souple

ISBN 10 : 1071628615 ISBN 13 : 9781071628614

Editeur : Humana, 2023

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This volume details novel and popular approaches to decipher the Ubiquitin Code. Chapters guide readers through ubiquitin chain complexity, methods to generate non-hydrolysable ubiquitin-NEDD8, a fully synthetic method for the linear synthesis of fluorescently labelled rhodamine LC3A and LC3B, a HECT ligase-mediated ubiquitylation in vitro, a RNAi-based screening to identify DUBs, a Yuh1 activity assay, a pull-down to isolate K48 and K63 chains using specific nanobodies, identification of NeDDylated protein based in a NEDD8^R74Kmutant, in vivo identification of NEDDylated proteins from liver diseases, UbL-ID method to identify ubiquitin-like modified proteins, a LC3/GABARAP capturing system, and methods to isolate peptides that are closely associated with proteasomes or trapped inside the core particle of proteasomes in eukaryotic cells, among others. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, The Ubiquitin Code aims to to be a useful practical guide to researches to help further their study in this field.

Les informations fournies dans la section � Synopsis � peuvent faire r�f�rence � une autre �dition de ce titre.

�diteur: Humana
Date d'�dition: 2023
Langue: anglais
ISBN 10: 1071628615
ISBN 13: 9781071628614
Reliure: Broch�
Nombre de pages: 260
�diteur: Rodriguez Manuel S, Barrio Rosa
Coordonn�es du fabricant: non disponible
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The Ubiquitin Code (Methods in Molecular Biology)

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The Ubiquitin Code

Rosa Barrio

Edit� par Springer US Nov 2023, 2023

ISBN 10 : 1071628615 ISBN 13 : 9781071628614

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Taschenbuch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume details novel and popular approaches to decipher theUbiquitin Code. Chapters guide readers through ubiquitin chain complexity,methods to generate non-hydrolysable ubiquitin-NEDD8,afully synthetic method for the linear synthesis of fluorescently labelled rhodamine LC3A and LC3B,aHECTligase-mediated ubiquitylationin vitro,aRNAi-based screeningto identify DUBs,aYuh1 activityassay,a pull-down to isolateK48 and K63 chainsusingspecific nanobodies,identification of NeDDylated protein based in aNEDD8R74Kmutant,in vivoidentificationof NEDDylated proteins fromliver diseases,UbL-ID method to identifyubiquitin-like modifiedproteins,a LC3/GABARAP capturing system, andmethods to isolate peptides that are closely associated with proteasomes or trapped inside the core particle of proteasomes in eukaryotic cells,among others.Written in the successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge,The Ubiquitin Codeaimstoto be auseful practical guide to researches to help further their study in this field. 260 pp. Englisch. N� de r�f. du vendeur 9781071628614

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The Ubiquitin Code

Rosa Barrio

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Taschenbuch. Etat : Neu. This item is printed on demand - Print on Demand Titel. Neuware -This volume details novel and popular approaches to decipher the Ubiquitin Code. Chapters guide readers through ubiquitin chain complexity, methods to generate non-hydrolysable ubiquitin-NEDD8, a fully synthetic method for the linear synthesis of fluorescently labelled rhodamine LC3A and LC3B, a HECT ligase-mediated ubiquitylation in vitro, a RNAi-based screening to identify DUBs, a Yuh1 activity assay, a pull-down to isolate K48 and K63 chains using specific nanobodies, identification of NeDDylated protein based in a NEDD8R74K mutant, in vivo identification of NEDDylated proteins from liver diseases, UbL-ID method to identify ubiquitin-like modified proteins, a LC3/GABARAP capturing system, and methods to isolate peptides that are closely associated with proteasomes or trapped inside the core particle of proteasomes in eukaryotic cells, among others. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, The Ubiquitin Code aims to to be a useful practical guide to researches to help further their study in this field.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 260 pp. Englisch. N� de r�f. du vendeur 9781071628614

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The Ubiquitin Code

Rosa Barrio

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ISBN 10 : 1071628615 ISBN 13 : 9781071628614

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Taschenbuch. Etat : Neu. Druck auf Anfrage Neuware - Printed after ordering - This volume details novel and popular approaches to decipher theUbiquitin Code. Chapters guide readers through ubiquitin chain complexity,methods to generate non-hydrolysable ubiquitin-NEDD8,afully synthetic method for the linear synthesis of fluorescently labelled rhodamine LC3A and LC3B,aHECTligase-mediated ubiquitylationin vitro,aRNAi-based screeningto identify DUBs,aYuh1 activityassay,a pull-down to isolateK48 and K63 chainsusingspecific nanobodies,identification of NeDDylated protein based in aNEDD8R74Kmutant,in vivoidentificationof NEDDylated proteins fromliver diseases,UbL-ID method to identifyubiquitin-like modifiedproteins,a LC3/GABARAP capturing system, andmethods to isolate peptides that are closely associated with proteasomes or trapped inside the core particle of proteasomes in eukaryotic cells,among others.Written in the successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge,The Ubiquitin Codeaimstoto be auseful practical guide to researches to help further their study in this field. N� de r�f. du vendeur 9781071628614

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The Ubiquitin Code

Manuel S Rodriguez

Edit� par Springer-Verlag New York Inc., 2023

ISBN 10 : 1071628615 ISBN 13 : 9781071628614

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