1. Dynamic SILAC to Determine Protein Turnover in Neurons and Glia
Aline R. Dörrbaum, Erin M. Schuman, and Julian D. Langer
2. SILAC-Based Proteomic Analysis of Meiosis in the Fission Yeast Schizosaccharomyces pombe
Dorothea Anrather, Silvia Bagelova Polakova, Lubos Cipak, and Juraj Gregan
3. Identification of Protein Interaction Partners in Bacteria Using Affinity Purification and SILAC Quantitative Proteomics
Monika Kopeckova, Marek Link, and Ivona Pavkova
4. pSILAC-Based Determination of Cellular Protein Sorting into Extracellular Vesicles
So-Fong Cam Ngan, Neil E. McCarthy, and Siu Kwan Sze
5. Quantification of Protein Palmitoylation by Cysteine-SILAC
Caiyun Fang, Xiaoqin Zhang, and Haojie Lu
6. Comparative SUMO Proteome Analysis Using Stable Isotopic Labeling by Amino Acids (SILAC)
Georgia Chachami and Sina-Victoria Barysch
7. A Super-SILAC Approach for Profiling Histone Post-Translational Modifications
Roberta Noberini, Elisa Longhi, and Tiziana Bonaldi
8. SILAC-Based Quantitative Phosphoproteomics in Yeast
María Luisa Hernáez and Concha Gil
9. Phosphotyrosine Profiling Using SILAC
Keshava K. Datta, Aditi Chatterjee, and Harsha Gowda
10. Chemoproteomic Profiling of Geranyl Pyrophosphate-Binding Proteins
Rong Cai and Yinsheng Wang
11. Quantitative Analysis of the Endoplasmic Reticulum-Associated Proteins Using ER-Localizable Reactive Molecules
Tomonori Tamura and Itaru Hamachi
12. Workflow for Quantitative Proteomic Analysis of Intestinal Organoids Using SILAC
Alexis Gonneaud, Claude Asselin, Véronique Giroux, and François-Michel Boisvert
13. Stable Isotope Labeling by Amino Acids and Bioorthogonal Noncanonical Amino Acid Tagging in Cultured Primary Neurons
Guoan Zhang, Katrin Deinhardt, and Thomas A. Neubert
14. Heavy Methyl SILAC Metabolic Labeling of Human Cell Lines for High-Confidence Identification of R/K-Methylated Peptides by High-Resolution Mass Spectrometry
Enrico Massignani, Marianna Maniaci, and Tiziana Bonaldi
15. SILAC-Based Quantitative Proteomic Analysis of Drosophila Embryos
Alistair Langlands, Hamze Beati, and H.- Arno J. Müller
16. Super-SILAC Quantitative Proteome Profiling of Zebrafish Larvae
Guillermo Aragoneses-Cazorla, Andres Machuca, M. Pilar Buendia-Nacarino, Daniela S. Anunciação, Estefania Garcia-Calvo, and Jose L. Luque-Garcia
Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.
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Hardcover. Etat : new. Hardcover. This detailed volume explores the most recent methodologies and applications of stable isotope labeling by amino acids in cell culture (SILAC). With the development of new technologies in the field of mass spectrometry and bioinformatics, SILAC-based methods have become the first tool of choice in quantitative proteomics either on its own or in combination with other more recently developed approaches, which is elucidated in the chapters of this book. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, SILAC: Methods and Protocols serves as an ideal guide for researchers working in the area of functional proteomics and other aspects of protein science. With the development of new technologies in the field of mass spectrometry and bioinformatics, SILAC-based methods have become the first tool of choice in quantitative proteomics either on its own or in combination with other more recently developed approaches, which is elucidated in the chapters of this book. Shipping may be from multiple locations in the US or from the UK, depending on stock availability. N° de réf. du vendeur 9781071628621
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Buch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed volume explores the most recent methodologies and applications of stable isotope labeling by amino acids in cell culture (SILAC). With the development of new technologies in the field of mass spectrometry and bioinformatics, SILAC-based methods have become the first tool of choice in quantitative proteomics either on its own or in combination with other more recently developed approaches, which is elucidated in the chapters of this book. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, SILAC: Methods and Protocols serves as an ideal guide for researchers working in the area of functional proteomics and other aspects of protein science. 300 pp. Englisch. N° de réf. du vendeur 9781071628621
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