Synopsis
Part I: Overview of BEs, Diverse Variants of BEs, and Computational Tools for Bes
1. Introduction and Perspectives of DNA Base Editors
Beomjong Song and Sangsu Bae
2. Web-Based Computational Tools for Base Editors
Gue-Ho Hwang and Sangsu Bae
3. Prediction of Base Editing Efficiencies and Outcomes Using DeepABE and DeepCBE
Jinman Park and Hui Kwon Kim
4. Profiling Genome-Wide Specificity of dCpf1 Cytidine Base Editors Using Digenome-Seq
Daesik Kim
Part II: Application of BEs for Disease Modeling
5. Base Editing of Human Hematopoietic Stem Cells
Jing Zeng, Gabriele Casirati, My Anh Nguyen, Pietro Genovese, and Daniel E. Bauer
6. A/C Simultaneous Conversion Using the Dual Base Editor in Human Cells
Xiaohui Zhang, Yuting Guan, and Dali Li
7. Functional Analysis of Variants in BRCA1 Using CRISPR Base-Editors
Ji-Eun See and Yongsub Kim
8. Use of the Representative Base Editing Tool Target-AID to Introduce Pathogenic Mutations into Mice
Hiroki Sasaguri
9. Targeted Mutagenesis in Mice Using a Base Editor
Tae Yeong Jeong, Soo-Yeon Lim, Je Kyung Seong, and Kyoungmi Kim
Part III: Therapeutic Base Editing Strategies
10. Heterologous Expression and Purification of a CRISPR-Cas9-Based Adenine Base Editor
Seu-Na Lee, Hong-Su Jang, and Jae-Sung Woo
11. Delivering Base Editors In Vivo by Adeno-Associated Virus Vectors
Colin K.W. Lim, Angelo J. Miskalis, Pablo Perez-Pinera, and Thomas Gaj
12. The Delivery of ABE mRNA to the Adult Murine Liver by Lipid Nanoparticles (LNPs)
Dong Yang and Chun-Qing Song
13. Ex Vivo Base Editing Therapy with Chemically-Derived Hepatic Progenitors
Yohan Kim, Jaemin Jeong, and Dongho Choi
14. Application of Base Editor-Mediated Genome Editing in Mouse Retina
Jun Wu, Chang Sik Cho, Dong Hyun Jo, and Jeong Hun Kim
Part IV: Use of BEs in Plants and Bacteria
15. High-Throughput Base-Editing-Mediated Artificial Evolution Streamlines Trait Gene Identification in Rice
Fang Yan, Man Yu, Meixia Wang, and Huanbin Zhou
16. Heritable Virus-Induced Genome Editing (VIGE) in Nicotiana attenuate
Hyeonjin Kim, Youngbin Oh, Eunae Park, Moonyoung Kang, Yuri Choi, and Sang-Gyu Kim
17. Cytosine Base Editing in Bacteria
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Base Editors: Methods and Protocols (Methods in Molecular Biology, 2606)
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Etat : New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. This volume explores base editors (BEs), an invaluable CRISPR-based genome editing tool with a wide variety of versatile applications. Beginning with an overview of BEs, their diverse variants, and computational tools, the book continues with experimental a. N° de réf. du vendeur 697759042
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Buch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume explores base editors (BEs), an invaluable CRISPR-based genome editing tool with a wide variety of versatile applications. Beginning with an overview of BEs, their diverse variants, and computational tools, the book continues with experimental applications of BEs for disease modeling in mammalian cells and generating mutagenic mice, therapeutic base editing strategies, which covers delivery methods of BE-encoded DNA plasmids, mRNAs, or ribonucleoproteins through viruses or non-viral lipid nanoparticles, and lastly, the use of BEs in plants and bacteria. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Base Editors: Methods and Protocols serves as an ideal guide for researchers looking to use base editors to continue their studies in an array of fields. 248 pp. Englisch. N° de réf. du vendeur 9781071628782
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Buch. Etat : Neu. Base Editors | Methods and Protocols | Beomjong Song (u. a.) | Buch | xii | Englisch | 2023 | Springer US | EAN 9781071628782 | Verantwortliche Person für die EU: Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg, juergen[dot]hartmann[at]springer[dot]com | Anbieter: preigu Print on Demand. N° de réf. du vendeur 123647167
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Buch. Etat : Neu. Neuware -This volume explores base editors (BEs), an invaluable CRISPR-based genome editing tool with a wide variety of versatile applications. Beginning with an overview of BEs, their diverse variants, and computational tools, the book continues with experimental applications of BEs for disease modeling in mammalian cells and generating mutagenic mice, therapeutic base editing strategies, which covers delivery methods of BE-encoded DNA plasmids, mRNAs, or ribonucleoproteins through viruses or non-viral lipid nanoparticles, and lastly, the use of BEs in plants and bacteria. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 248 pp. Englisch. N° de réf. du vendeur 9781071628782
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Buch. Etat : Neu. Druck auf Anfrage Neuware - Printed after ordering - This volume explores base editors (BEs), an invaluable CRISPR-based genome editing tool with a wide variety of versatile applications. Beginning with an overview of BEs, their diverse variants, and computational tools, the book continues with experimental applications of BEs for disease modeling in mammalian cells and generating mutagenic mice, therapeutic base editing strategies, which covers delivery methods of BE-encoded DNA plasmids, mRNAs, or ribonucleoproteins through viruses or non-viral lipid nanoparticles, and lastly, the use of BEs in plants and bacteria. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Base Editors: Methods and Protocols serves as an ideal guide for researchers looking to use base editors to continue their studies in an array of fields. N° de réf. du vendeur 9781071628782
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