Chromatin Accessibility: Methods and Protocols - Couverture rigide

Couverture rigide

ISBN 10 : 1071628984 ISBN 13 : 9781071628980

Editeur : Humana, 2023

Synopsis

This detailed volume provides a comprehensive resource covering the existing and state-of-the-art tools in the field of profiling chromatin accessibility and its dynamics. Beginning with a section on bulk-cell methods for profiling chromatin accessibility and nucleosome positioning that rely on enzymatic cleavage of accessible DNA and produce information about relative accessibility, the book continues with methods that use single-molecule and enzymatic approaches to solving the problem of mapping absolute occupancy/accessibility, emerging tools for mapping DNA accessibility and nucleosome positioning in single cells, imaging-based methods for visualizing accessible chromatin in its nuclear context, as well as computational methods for the processing and analysis of chromatin accessibility datasets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and up-to-date, Chromatin Accessibility: Methods and Protocols serves as an extensive and useful reference for researchers studying different facets of chromatin accessibility in a wide variety of biological contexts. 
Chapter 6 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Détails de l'édition

• Éditeur: Humana
• Date d'édition: 2023
• Langue: anglais
• ISBN 10: 1071628984
• ISBN 13: 9781071628980
• Reliure: Relié
• Nombre de pages: 352
• Éditeur: Marinov Georgi K., Greenleaf William J.
• Coordonnées du fabricant: Springer Nature Customer Service Center GmbH; ProductSafety@springernature.com; ; Europaplatz 3; Heidelberg; 69115; Allemagne