Lipidomics: Methods and Protocols - Couverture rigide

Couverture rigide

ISBN 10 : 1071629654 ISBN 13 : 9781071629659

Editeur : Humana, 2023

Synopsis

1. Isolation of Mitochondrial Lipids and Mass Spectrometric Analysis

Alexa M. Jauregui, Zoé M. Cubero Cortés, Sean D. Meehan, and Sanjoy K. Bhattacharya

2. Isolation of Neuronal Synaptic Membranes by Sucrose Gradient Centrifugation

Blake R. Hopiavuori, Dustin R. Masser, Joseph L. Wilkerson, Richard S. Brush, Nawajes A. Mandal, Robert E. Anderson, and Willard M. Freeman

3. Multi-Macromolecular Extraction from Endosymbiotic Anthozoans

Anderson B. Mayfield

4. High Resolution Liquid Chromatography-Mass Spectrometry for Lipidomics

Faith Christine Harvey, Vanessa Collao, and Sanjoy K. Bhattacharya

5. Characterization of Gangliosides from Mouse Optic Nerve Samples Using Mass Spectrometry

Jennifer Arcuri and Sanjoy K. Bhattacharya

6. Protocol and Methods Applicable to Retinal Vascular Diseases

Saurabh Kumar, Manjunath B. Joshi, and Inderjeet Kaur

7. Analysis and Annotation of Phospholipids by Mass Spectrometry-Based Metabolomics

Álvaro González-Domínguez, María Santos-Martín, Ana Sayago, Alfonso María Lechuga-Sancho, Ángeles Fernández-Recamales, and Raúl González-Domínguez

8. Profiling the Mammalian Lipidome by Quantitative Shotgun Lipidomics

Mads Møller Foged, Kenji Maeda, and Mesut Bilgin

9. Lipidomics Analysis with Triple Quadrupole Mass Spectrometry

Meher A. Saleem, Anna Mueller, and Sanjoy K. Bhattacharya

10. High-Performance Chromatographic Separation of Cerebrosides

Renaud Sicard and Ralf Landgraf

11. Quantification of Endocannabinoids from Biological Samples Using Liquid Chromatography Tandem Mass Spectrometry

Zhiying Wang, Chenglin Mo, Lynda Bonewald, and Marco Brotto

12. Analysis of Lipids, Fatty Acid, and Cholesterol in Membrane Microdomains

Martin-Paul Agbaga, Mark E. McClellan, and Michael H. Elliott

13. Analysis of Cholesterol Lipids Using Gas Chromatography Mass Spectrometry

Emily J. Neag, Vanessa Collao, and Sanjoy K. Bhattacharya

14. Analyses and Localization of Phosphatidylcholine and Phosphatidylserine in Murine Ocular Tissue Using Imaging Mass Spectrometry

Varun Krishnan, Sean D. Meehan, Colin Hayter, and Sanjoy K. Bhattacharya

15. A Robust Phenotypic Screening Assay Utilizing Human Podocytes to Identify Agents that Modulate Lipid Droplets

Margaret Gurumani, Shamroop Mallela, Javier Varona, Sandra Merscher, Alessia Fornoni, and Hassan Al-Ali

16. Capillary Electrophoresis Plasma Fractionation for Lipids Analysis

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A propos du livre

À propos de la quatrième de couverture

This fully updated book presents an account of areas of utility, techniques, and bioinformatic advancements in the field of lipidomics. Beginning with protocols for lipid isolation and extraction, the volume continues with techniques from extractive mass spectrometry to imaging mass spectrometry methods allowing localization of lipids in tissues. These protocols have been complemented by methods addressing specific problems from membranes, fractionated subcellular compartments or organelles to whole organisms. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Lipidomics: Methods and Protocols, Second Edition serves as an ideal guide for biochemists, molecular biologists, neuroscientists, vision research scientists, as well as all biomedical researchers with interest in disease discovery and drug development.

Détails de l'édition

• Éditeur: Humana
• Date d'édition: 2023
• Langue: anglais
• ISBN 10: 1071629654
• ISBN 13: 9781071629659
• Reliure: Relié
• Numéro d'édition: 2
• Nombre de pages: 374
• Éditeur: Bhattacharya Sanjoy K
• Coordonnées du fabricant: Springer Nature Customer Service Center GmbH; ProductSafety@springernature.com; ; Europaplatz 3; Heidelberg; 69115; Allemagne