1. The Vast Utility of Drosophila Oogenesis
Michelle S. Giedt and Tina L. Tootle
2. Visualization and Quantification of Drosophila Larval Ovaries
Alicia E. Rosales-Nieves, Miriam Marín-Menguiano, Alejandro Campoy-Lopez, and Acaimo González-Reyes
3. Dissection, Fixation, and Standard Staining of Adult Drosophila Ovaries
Julie A. Merkle
4. Utilizing the FLP-Out System for Clonal RNAi Analysis in the Adult Drosophila Ovary
Daniel N. Phipps, Amanda M. Powell, and Elizabeth T. Ables
5. Analysis of Physiological Control of Adult Drosophila Oogenesis by Inter-Organ Communication
Lesley N. Weaver
6. Immunohistochemical Analysis of Nuclear Lamina Structures in the Drosophila Ovary Using CRISPR-Tagged Genes
Tingting Duan, Felipe Rodriguez-Tirado, and Pamela K. Geyer
7. Visualizing Fusome Morphology via Tubulin Immunofluorescence in Drosophila Ovarian Germ Cells
Anna E. Williams and Elizabeth T. Ables
8. Tracking Follicle Cell Development
Adrianna Soriano, Christopher Petit, Savannah Ryan, and Jennifer C. Jemc
9. Optimized Fixation and Phalloidin Staining of Basally Localized F-Actin Networks in Collectively Migrating Follicle Cells
Mitchell T. Anderson, Kristin Sherrard, and Sally Horne-Badovinac
10. Quantitative Image Analysis of Dynamic Cell Behaviors during Border Cell Migration
Yujun Chen, Nirupama Kotian, and Jocelyn A. McDonald
11. Live Imaging of Nurse Cell Behavior in Late Stages of Drosophila Oogenesis
Jonathan A. Jackson, Jasmin Imran Alsous, and Adam C. Martin
12. Visualizing Lipid Droplets in Drosophila Oogenesis
Roger P. White and Michael A. Welte
13. Assessing Ovulation in Drosophila melanogaster
Andrew Beard, Rebecca Oramas, and Jianjun Sun
14. A Low-Tech Flow Chamber for Live Imaging of Drosophila Egg Chambers during Drug Treatments
Allison L. Zajac, Audrey M. Williams, and Sally Horne-Badovinac
15. Detection and Assessment of Wolbachia pipientis Infection
Lindsay B.M. Nevalainen and Irene L.G. Newton
16. Measuring Transposable Element Activity in Adult Drosophila Ovaries
Anikó Szabó, Péter Borkúti, Zoltán Kovács, Ildikó Kristó, Csilla Abonyi, and Péter Vilmos
17. Preparation of Drosophila Ovarioles for Single-Cell RNA Sequencing
N
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Gebunden. Etat : New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Includes cutting-edge techniquesProvides step-by-step detail essential for reproducible resultsContains key implementation advice from the expertsThis detailed volume compiles numerous methods to explore fly oogenesis. Beginning wit. N° de réf. du vendeur 738835611
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Buch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed volume compiles numerous methods to explore fly oogenesis. Beginning with updated protocols from isolating and staining the ovary to numerous imaging techniques and genetic protocols for cell-specific assessment and CRISPR-mediated mutagenesis, the volume continues by detailing techniques from assessing cytoskeletal structures to uncovering protein-protein interactions, with closing chapters exploring how Drosophila oogenesis can be used in the classroom and in outreach programs to increase interest in biomedical research, STEM education, and STEM careers. Written for the highly successfulMethods in Molecular Biologyseries, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical,Drosophila Oogenesis: Methods and Protocolsserves as an ideal guide for both new and expert researchers working with this robust and versatile model organism. 464 pp. Englisch. N° de réf. du vendeur 9781071629697
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Buch. Etat : Neu. This item is printed on demand - Print on Demand Titel. Neuware -The Vast Utility of Drosophila Oogenesis.- Visualization and Quantification of Drosophila Larval Ovaries.- Dissection, Fixation, and Standard Staining of Adult Drosophila Ovaries.- Utilizing the FLP-Out System for Clonal RNAi Analysis in the Adult Drosophila Ovary.- Analysis of Physiological Control of Adult Drosophila Oogenesis by Inter-Organ Communication.- Immunohistochemical Analysis of Nuclear Lamina Structures in the Drosophila Ovary Using CRISPR-Tagged Genes.- Visualizing Fusome Morphology via Tubulin Immunofluorescence in Drosophila Ovarian Germ Cells.- Tracking Follicle Cell Development.- Optimized Fixation and Phalloidin Staining of Basally Localized F-Actin Networks in Collectively Migrating Follicle Cells.- Quantitative Image Analysis of Dynamic Cell Behaviors during Border Cell Migration.- Live Imaging of Nurse Cell Behavior in Late Stages of Drosophila Oogenesis.- Visualizing Lipid Droplets in Drosophila Oogenesis.- Assessing Ovulation in Drosophila melanogaster.- A Low-Tech Flow Chamber for Live Imaging of Drosophila Egg Chambers during Drug Treatments.- Detection and Assessment of Wolbachia pipientis Infection.- Measuring Transposable Element Activity in Adult Drosophila Ovaries.- Preparation of Drosophila Ovarioles for Single-Cell RNA Sequencing.- Chromatin Immunoprecipitation Experiments from Drosophila Ovaries.- Detection of Actin in Nuclear Protein Fraction Isolated from Adult Drosophila Ovary.- STAMP: Spatio-Temporal Association Mapping of Proteins.- Using Drosophila Oogenesis in the Classroom to Increase Student Participation in Biomedical Research.- Gaining Wings to FLY: Using Drosophila Oogenesis as an Entry Point for Citizen Scientists in Laboratory Research.Libri GmbH, Europaallee 1, 36244 Bad Hersfeld 464 pp. Englisch. N° de réf. du vendeur 9781071629697
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Buch. Etat : Neu. Druck auf Anfrage Neuware - Printed after ordering - This detailed volume compiles numerous methods to explore fly oogenesis. Beginning with updated protocols from isolating and staining the ovary to numerous imaging techniques and genetic protocols for cell-specific assessment and CRISPR-mediated mutagenesis, the volume continues by detailing techniques from assessing cytoskeletal structures to uncovering protein-protein interactions, with closing chapters exploring how Drosophila oogenesis can be used in the classroom and in outreach programs to increase interest in biomedical research, STEM education, and STEM careers. Written for the highly successfulMethods in Molecular Biologyseries, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical,Drosophila Oogenesis: Methods and Protocolsserves as an ideal guide for both new and expert researchers working with this robust and versatile model organism. N° de réf. du vendeur 9781071629697
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