This second edition details new and updated protocols that cover techniques used to study secretion systems. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and the role of energy during assembly and/or secretion, identifying secreted effectors as well as using reporters to follow effector transport. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Bacterial Secretion Systems: Methods and Protocols- Second Edition aims to be a useful andpractical guide to new researchers and experts looking to expand their knowledge.
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Etat : New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Includes cutting-edge methods and protocolsProvides step-by-step detail essential for reproducible resultsContains key notes and implementation advice from the expertsThis second edition details new and updated protocols that cov. N° de réf. du vendeur 888374151
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Buch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This second edition details new and updated protocols that cover techniques used to study secretion systems. Chapters focus on identifying and localizingthe different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and the role of energy during assembly and/or secretion, identifying secreted effectors as well as using reporters to follow effector transport.Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge,Bacterial Secretion Systems: Methods and Protocols- Second Editionaimsto be auseful and practical guide to new researchersand experts looking to expand their knowledge. 632 pp. Englisch. N° de réf. du vendeur 9781071634448
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Buch. Etat : Neu. Bacterial Secretion Systems | Methods and Protocols | Eric Cascales (u. a.) | Buch | xvi | Englisch | 2023 | Springer US | EAN 9781071634448 | Verantwortliche Person für die EU: Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg, juergen[dot]hartmann[at]springer[dot]com | Anbieter: preigu Print on Demand. N° de réf. du vendeur 127155931
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