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Synopsis
This detailed volume explores techniques for researching the diverse and specialized mechanisms for mRNA degradation, both in the cytoplasm and the nucleus. From classical methods for studying RNA degradation at the single RNA level to the latest transcriptome-wide approaches involving long-read sequencing and metabolic labeling, this book focuses on methods for eukaryotic models, such as procedures for studying deadenylation, decapping and exoribonuclease activity, assessing RNA decay rate, characterizing RNA degradation intermediates, RNA-proteins interactions, and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, mRNA Decay: Methods and Protocols provides both new and experienced RNA researchers with an inspiring collection of protocols to prompt further investigation of these vital degradation pathways.
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Mrna Decay : Methods and Protocols
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Hardcover. Etat : new. Hardcover. This detailed volume explores techniques for researching the diverse and specialized mechanisms for mRNA degradation, both in the cytoplasm and the nucleus. From classical methods for studying RNA degradation at the single RNA level to the latest transcriptome-wide approaches involving long-read sequencing and metabolic labeling, this book focuses on methods for eukaryotic models, such as procedures for studying deadenylation, decapping and exoribonuclease activity, assessing RNA decay rate, characterizing RNA degradation intermediates, RNA-proteins interactions, and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, mRNA Decay: Methods and Protocols provides both new and experienced RNA researchers with an inspiring collection of protocols to prompt further investigation of these vital degradation pathways. Shipping may be from multiple locations in the US or from the UK, depending on stock availability. N° de réf. du vendeur 9781071641750
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Hardcover. Etat : new. Hardcover. This detailed volume explores techniques for researching the diverse and specialized mechanisms for mRNA degradation, both in the cytoplasm and the nucleus. From classical methods for studying RNA degradation at the single RNA level to the latest transcriptome-wide approaches involving long-read sequencing and metabolic labeling, this book focuses on methods for eukaryotic models, such as procedures for studying deadenylation, decapping and exoribonuclease activity, assessing RNA decay rate, characterizing RNA degradation intermediates, RNA-proteins interactions, and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, mRNA Decay: Methods and Protocols provides both new and experienced RNA researchers with an inspiring collection of protocols to prompt further investigation of these vital degradation pathways. Shipping may be from our UK warehouse or from our Australian or US warehouses, depending on stock availability. N° de réf. du vendeur 9781071641750
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