Botrytis cinerea: Methods and Protocols - Couverture rigide

9781071655504: Botrytis cinerea: Methods and Protocols

Couverture rigide

ISBN 10 : 1071655507 ISBN 13 : 9781071655504

Editeur : Humana, 2026

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This volume provides comprehensive protocols on Botrytis cinerea and the main methods used to study this important fungal species. Chapters detail cover classical approaches, including isolation, cultural techniques, sclerotia induction, storage, microscopy and protein localization, inoculation assays on fruits and vegetables, antifungal susceptibility testing, protoplast production and transfection, RNA interference, and identification of mycoviral infections. Additionally, the volume presents state-of-the-art molecular and omics-based approaches, including high-quality DNA and RNA extraction, molecular marker development, genome assembly, quantitative gene expression analysis, and integrative transcriptomic, proteomic, and metabolomic analyses. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

Authoritative and comprehensive, Botrytis cinerea: Methods and Protocols aims to promote experimental rigor, facilitate cross-disciplinary collaboration, and stimulate innovative research directions in Botrytis biology.

Les informations fournies dans la section � Synopsis � peuvent faire r�f�rence � une autre �dition de ce titre.

�diteur: Humana
Date d'�dition: 2026
Langue: anglais
ISBN 10: 1071655507
ISBN 13: 9781071655504
Reliure: Reli�
Nombre de pages: 216
�diteur: Nerva Luca, Chitarra Walter
Coordonn�es du fabricant: Springer Nature Customer Service Center GmbH
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Botrytis Cinerea

Luca Nerva

Edit� par Springer Us Nov 2026, 2026

ISBN 10 : 1071655507 ISBN 13 : 9781071655504

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Buch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume provides comprehensive protocols on Botrytis cinerea and the main methods used to study this important fungal species. Chapters detail cover classical approaches, including isolation, cultural techniques, sclerotia induction, storage, microscopy and protein localization, inoculation assays on fruits and vegetables, antifungal susceptibility testing, protoplast production and transfection, RNA interference, and identification of mycoviral infections. Additionally, the volume presents state-of-the-art molecular and omics-based approaches, including high-quality DNA and RNA extraction, molecular marker development, genome assembly, quantitative gene expression analysis, and integrative transcriptomic, proteomic, and metabolomic analyses. Written for the highly successfulMethods in Molecular Biologyseries, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.Authoritative and comprehensive,Botrytis cinerea: Methods and Protocols aims to promote experimental rigor, facilitate cross-disciplinary collaboration, and stimulate innovative research directions in Botrytis biology. 204 pp. Englisch. N� de r�f. du vendeur 9781071655504

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