RNA-Chromatin Interactions: Methods and Protocols - Couverture rigide

Couverture rigide

ISBN 10 : 1071656260 ISBN 13 : 9781071656266

Editeur : Humana, 2026

Synopsis

This second edition volume expands on the previous edition with an update on the latest methods used to study next-generation sequencing for large-scale analysis of nascent RNA and chromatin features associated with RNA function, as well as advanced techniques for functional studies of non-coding RNA function in the nucleus. The chapters in this book cover topics such as using BruUV-seq and TT-seq to identify enhancer RNA expression in live cells; revealing high-resolution open chromatin-associated interactions by NicE-C; purification of RNA G-quadruplex binding proteins; examining RNA modifications on chromatin or formation of R-loops; measuring the proximity of nuclear lncRNAs to nuclear bodies; and Cas13d-mediated knockdown of long non-coding RNAs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Timely and comprehensive, RNA-Chromatin Interactions: Methods and Protocols, Second Edition is a valuable resource for new and experienced researchers in the RNA-chromatin field who want to expand their work to uncover new and exciting molecular mechanisms in both basic research and more disease-focused work.

 

Détails de l'édition

• Éditeur: Humana
• Date d'édition: 2026
• Langue: anglais
• ISBN 10: 1071656260
• ISBN 13: 9781071656266
• Reliure: Relié
• Numéro d'édition: 2
• Nombre de pages: 320
• Éditeur: Ørom Ulf Andersson Vang, Li Wenbo, Lee Joo-Hyung
• Coordonnées du fabricant: Springer Nature Customer Service Center GmbH; ProductSafety@springernature.com; ; Europaplatz 3,69115 Heidelberg, Germany; Heidelberg; 69115; Allemagne