PCR Technology - Couverture souple

Couverture souple

ISBN 10 : 1138198587 ISBN 13 : 9781138198586

Editeur : CRC Press, 2016

Synopsis

A huge amount of innovation surrounds PCR as a molecular technique, as researchers continually think of new ways to tweak, adapt, and re-formulate concepts and applications. This volume provides a critical reference point for anyone wishing to use this technology. Topics include the purification and handling of PCR templates, the effect of the manufacture and purification of the oligonucleotide on PCR behavior, optimum buffer composition, probe options, and the design and optimization of qPCR assays. The book also explores the development and refinement of instrumentation and effective controls to protect against uncertainties due to reaction variability.

A propos du livre

À propos de l?auteur

Dr. Tania Nolan is an internationally recognized molecular biologist with a reputation for expertise in the field of mRNA quantification using RT-qPCR. She was a significant coauthor of the specialist textbook The A-Z of Quantitative PCR along with numerous other book chapters and peer-reviewed papers. She has presented several plenary lectures and chaired sessions at major international qPCR meetings.

Stephen A. Bustin, BS(Mod) PhD FSB acquired his first qPCR instrument in 1997 and has published numerous peer-reviewed papers that describe and use this technology. He wrote and edited the A-Z of Quantitative PCR, universally acknowledged as the "qPCR Bible," edited The PCR Revolution, and has written a series of Definitive qPCR ebooks. He also led the international consortium that drew up the MIQE guidelines.

Détails de l'édition

• Éditeur: CRC Press
• Date d'édition: 2016
• Langue: anglais
• ISBN 10: 1138198587
• ISBN 13: 9781138198586
• Reliure: Broché
• Numéro d'édition: 3
• Nombre de pages: 470
• Éditeur: Nolan Tania, Bustin Stephen A.
• Coordonnées du fabricant: non disponible
• Personne responsable: non disponible