Synopsis
I: General Methodologies.- 1: Kinetics of Transport: Characterizing the Interaction of Substrates and Inhibitors with Carrier Systems.- 2: Carrier Kinetics Show How the Sodium Pump Uses ATP to Render Pumping of Both Sodium and Potassium Effectively and Suggest a Model for the Action of the F0F1 ATP-ases.- 3: Voltage-Dependent Conductances: Gating Currents and Single-Channel Recordings.- 4: Methods of Determining the Routes of Intestinal Water Transport.- 2: Isotopic Methods for Flux Determination in Various Membrane Preparations.- 5: Characterization of Transport Systems for Solutes at the Blood Side of Endothelial and Parenchymal Cells by Single Circulation Paired Tracer Dilution. A Review of Recent Studies.- 6: Some Inexact Methods for the Analysis of Transport Kinetics Using Dual Tracers.- 7: Transport Studies in Isolated Enterocytes.- 8: Cell Culture in the Study of Epithelial Development and Function.- 9: New Approach to the Study of Transport of Biomolecules by Microcarrier Cell Cultures Perfused in a Column Applying a High Resolution Paired-Tracer Technique.- 10: Methodological Guide for Studying Epithelial Transport with Isolated Membrane Vesicles.- III: Electrophysiological Methods.- 11: The Use of Ion-Selective Microelectrodes to Study Cellular Transport Processes.- 12: Voltage-Dependent Ionic Conductances: "Whole-Cell" Recording by Patch-Clamp Techniques.- 13: Electrophysiological Methods in the Study of Water Transport across Cell Membranes.- IV: Spectroscopic Methods.- 14: A Practical Introduction to the Use of Intracellular Fluorescent Indicators.- 15: Biomedical NMR Spectroscopy and Transport.- 16: Proton NMR Studies of Transmembrane Solute Transport.- 17: Optical and NMR Spectroscopy in the Study of Secretion.- V: Molecular Approaches to Structure and Function of Transporters.- 18: Anion Transport Systems: Continuous Monitoring of Transport by Fluorescence (CMTF) in Cells and Vesicles.- 19: The Molecular Biology of Sugar Transport Proteins.- 20: Chemical and Molecular Probes of Nucleoside Transport Mechanisms in Mammalian Tissues.- VI: Transport Regulation.- 21: Regulation of Junctional Intercellular Communication by Tyrosine-Protein Kinases. Role of the Cellular SRC Gene and Growth Factor Receptors.- 22: Single-Channel and Whole-Cell Patch-Clamp Experiments on Gland Cells: Activation of Ion Channels via Second Messengers.- 23: Application of Rapid Dual Tracer Dilution Techniques for the Study of Endothelial Cell Amino Acid Transport in Perfused Microcarrier Cultures.- Contributors.
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