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9781489943835: New Nucleic Acid Techniques

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ISBN 10 : 1489943838 ISBN 13 : 9781489943835

Editeur : Humana, 2013

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�diteur: Humana
Date d'�dition: 2013
Langue: anglais
ISBN 10: 1489943838
ISBN 13: 9781489943835
Reliure: Broch�
Nombre de pages: 580
�diteur: Walker John M.
Coordonn�es du fabricant: ProductSafety@springernature.com
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New Nucleic Acid Techniques

John M. Walker

Edit� par Humana Press Aug 2013, 2013

ISBN 10 : 1489943838 ISBN 13 : 9781489943835

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Taschenbuch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Electrophoresis of RNA Denatured with Glyoxal or Formaldehyde.- Northern Blotting.- Hybrid-Release Translation.- Hybrid-Arrested Translation.- In Vitro Translation and Analysis of Early Events in Protein Synthesis Initiation in Nonreticulocyte Mammalian Cells.- Use of Elutips to Purify DNA.- Plasmid Preparation on Sephacryl S1000.- Electrophoresis of DNA in Nondenaturing Polyacrylamide Gels.- Use of 35S Nucleotides for DNA Sequencing.- Direct Dideoxy DNA Sequencing.- Computer Applications to Molecular Biology.- Detection of Sequence-Specific Protein-DNA Interactions by the DNA-Footprinting Technique.- Oligonucleotide Synthesis Using the Manual Phosphotriester Method.- Manual Oligonucleotide Synthesis Using the Phosphoramidite Method.- Purification of Synthetic Oligonucleotides by Preparative Gel Electrophoresis.- Isolation of High Molecular Weight DNA Suitable for the Construction of Genomic Libraries.- Construction of Mammalian Genomic Libraries Using Replacement Vectors.- Cosmid Library Construction.- Construction of cDNA Libraries in gt10 or gt11.- Immunoscreening of gt11 Expression Libraries Using an Avidin�Biotin Detection System.- Primed Synthesis and Direct Sequencing in the Isolation of cDNA Clones Using Short Oligonucleotides.- Constructing Expression cDNA Libraries Using Unphosphorylated Adaptors.- Expression Screening of cDNA Libraries in pEX.- Producing Antibodies of Predetermined Specificity from Escherichia coli Hybrid Proteins.- Epitope Mapping Using pEX.- DNA Transfection of Mammalian Cells Using Polybrene.- Expression of Foreign Genes in Mammalian Cells.- Dot-Blot Hybridization Method.- Detection of Unique or Low Copy Number DNA Sequences in Complex Genomes.- Photobiotin-Labeled DNA and RNA Hybridization Probes.- Enzyme-Labeled DNA Probes.- Luminescent Detection of Specific DNA Sequences.- Detection of DNA Sequences Using Biotinylated Probes.- Crosslinking of Single-Stranded DNA to Resins.- Extraction of RNA from Plants.- Isolation of Plant Nuclei.- In Vitro Transcription in Plant Nuclei.- Large-Scale Isolation of Ti Plasmid DNA.- Transformation and Regeneration of Dicotyledonous Plants.- Plant Protoplast Fusion.- Plant Tissue Culture.- Direct Gene Transfer into Plant Protoplasts.- Isolation of Wheat Chloroplasts and Chloroplast DNA.- DNA Labeling by Isolated Wheat Chloroplasts. 580 pp. Englisch. N� de r�f. du vendeur 9781489943835

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New Nucleic Acid Techniques

Walker, John M.

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ISBN 10 : 1489943838 ISBN 13 : 9781489943835

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Etat : New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Electrophoresis of RNA Denatured with Glyoxal or Formaldehyde.- Northern Blotting.- Hybrid-Release Translation.- Hybrid-Arrested Translation.- In Vitro Translation and Analysis of Early Events in Protein Synthesis Initiation in Nonreticulocyte Mammalian Cel. N� de r�f. du vendeur 4212266

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New Nucleic Acid Techniques (Methods in Molecular Biology, 4)

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New Nucleic Acid Techniques

John M. Walker

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New Nucleic Acid Techniques

. Ed(s): Walker, John M.

Edit� par Humana Press Inc., 2013

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Etat : New. Editor(s): Walker, John M. Series: Methods in Molecular Biology. Num Pages: 560 pages, 17 black & white illustrations, biography. BIC Classification: PSB. Category: (P) Professional & Vocational. Dimension: 255 x 178 x 30. Weight in Grams: 992. . 2013. Softcover reprint of the original 1st ed. 1988. Paperback. . . . . N� de r�f. du vendeur V9781489943835

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New Nucleic Acid Techniques

John M. Walker

Edit� par Humana Press, Humana Press Aug 2013, 2013

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Taschenbuch. Etat : Neu. This item is printed on demand - Print on Demand Titel. Neuware -Electrophoresis of RNA Denatured with Glyoxal or Formaldehyde.- Northern Blotting.- Hybrid-Release Translation.- Hybrid-Arrested Translation.- In Vitro Translation and Analysis of Early Events in Protein Synthesis Initiation in Nonreticulocyte Mammalian Cells.- Use of Elutips to Purify DNA.- Plasmid Preparation on Sephacryl S1000.- Electrophoresis of DNA in Nondenaturing Polyacrylamide Gels.- Use of 35S Nucleotides for DNA Sequencing.- Direct Dideoxy DNA Sequencing.- Computer Applications to Molecular Biology.- Detection of Sequence-Specific Protein-DNA Interactions by the DNA-Footprinting Technique.- Oligonucleotide Synthesis Using the Manual Phosphotriester Method.- Manual Oligonucleotide Synthesis Using the Phosphoramidite Method.- Purification of Synthetic Oligonucleotides by Preparative Gel Electrophoresis.- Isolation of High Molecular Weight DNA Suitable for the Construction of Genomic Libraries.- Construction of Mammalian Genomic Libraries Using Replacement Vectors.- Cosmid Library Construction.- Construction of cDNA Libraries in gt10 or gt11.- Immunoscreening of gt11 Expression Libraries Using an Avidin�Biotin Detection System.- Primed Synthesis and Direct Sequencing in the Isolation of cDNA Clones Using Short Oligonucleotides.- Constructing Expression cDNA Libraries Using Unphosphorylated Adaptors.- Expression Screening of cDNA Libraries in pEX.- Producing Antibodies of Predetermined Specificity from Escherichia coli Hybrid Proteins.- Epitope Mapping Using pEX.- DNA Transfection of Mammalian Cells Using Polybrene.- Expression of Foreign Genes in Mammalian Cells.- Dot-Blot Hybridization Method.- Detection of Unique or Low Copy Number DNA Sequences in Complex Genomes.- Photobiotin-Labeled DNA and RNA Hybridization Probes.- Enzyme-Labeled DNA Probes.- Luminescent Detection of Specific DNA Sequences.- Detection of DNA Sequences Using Biotinylated Probes.- Crosslinking of Single-Stranded DNA to Resins.- Extraction of RNA from Plants.- Isolation of Plant Nuclei.- In Vitro Transcription in Plant Nuclei.- Large-Scale Isolation of Ti Plasmid DNA.- Transformation and Regeneration of Dicotyledonous Plants.- Plant Protoplast Fusion.- Plant Tissue Culture.- Direct Gene Transfer into Plant Protoplasts.- Isolation of Wheat Chloroplasts and Chloroplast DNA.- DNA Labeling by Isolated Wheat Chloroplasts.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 580 pp. Englisch. N� de r�f. du vendeur 9781489943835

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New Nucleic Acid Techniques

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New Nucleic Acid Techniques

John M. Walker

Edit� par Humana Press, Humana Press, 2013

ISBN 10 : 1489943838 ISBN 13 : 9781489943835

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Taschenbuch. Etat : Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - Electrophoresis of RNA Denatured with Glyoxal or Formaldehyde.- Northern Blotting.- Hybrid-Release Translation.- Hybrid-Arrested Translation.- In Vitro Translation and Analysis of Early Events in Protein Synthesis Initiation in Nonreticulocyte Mammalian Cells.- Use of Elutips to Purify DNA.- Plasmid Preparation on Sephacryl S1000.- Electrophoresis of DNA in Nondenaturing Polyacrylamide Gels.- Use of 35S Nucleotides for DNA Sequencing.- Direct Dideoxy DNA Sequencing.- Computer Applications to Molecular Biology.- Detection of Sequence-Specific Protein-DNA Interactions by the DNA-Footprinting Technique.- Oligonucleotide Synthesis Using the Manual Phosphotriester Method.- Manual Oligonucleotide Synthesis Using the Phosphoramidite Method.- Purification of Synthetic Oligonucleotides by Preparative Gel Electrophoresis.- Isolation of High Molecular Weight DNA Suitable for the Construction of Genomic Libraries.- Construction of Mammalian Genomic Libraries Using Replacement Vectors.- Cosmid Library Construction.- Construction of cDNA Libraries in gt10 or gt11.- Immunoscreening of gt11 Expression Libraries Using an Avidin�Biotin Detection System.- Primed Synthesis and Direct Sequencing in the Isolation of cDNA Clones Using Short Oligonucleotides.- Constructing Expression cDNA Libraries Using Unphosphorylated Adaptors.- Expression Screening of cDNA Libraries in pEX.- Producing Antibodies of Predetermined Specificity from Escherichia coli Hybrid Proteins.- Epitope Mapping Using pEX.- DNA Transfection of Mammalian Cells Using Polybrene.- Expression of Foreign Genes in Mammalian Cells.- Dot-Blot Hybridization Method.- Detection of Unique or Low Copy Number DNA Sequences in Complex Genomes.- Photobiotin-Labeled DNA and RNA Hybridization Probes.- Enzyme-Labeled DNA Probes.- Luminescent Detection of Specific DNA Sequences.- Detection of DNA Sequences Using Biotinylated Probes.- Crosslinking of Single-Stranded DNA to Resins.- Extraction of RNA from Plants.- Isolation of Plant Nuclei.- In Vitro Transcription in Plant Nuclei.- Large-Scale Isolation of Ti Plasmid DNA.- Transformation and Regeneration of Dicotyledonous Plants.- Plant Protoplast Fusion.- Plant Tissue Culture.- Direct Gene Transfer into Plant Protoplasts.- Isolation of Wheat Chloroplasts and Chloroplast DNA.- DNA Labeling by Isolated Wheat Chloroplasts. N� de r�f. du vendeur 9781489943835

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New Nucleic Acid Techniques

Walker John M.

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ISBN 10 : 1489943838 ISBN 13 : 9781489943835

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