Histology Protocols - Couverture souple

Couverture souple

ISBN 10 : 1493956949 ISBN 13 : 9781493956944

Editeur : Humana, 2016

Synopsis

Somuchofwhatweknowaboutthepathogenesisofhumandiseasehascomefromthe systematic and careful study of histological material. Indeed, every internal medicine discipline has its landmark papers describing the clinico-pathological correlations. However, increasingly, it is molecular and cellular biology that provides the necessary mechanistic insights. For many years, it was thought that the two skill sets were mutually exclusive, but we hope that this book shows that this is not necessarily so. Implicitinthescienceofhistologyisthepreservationandarchivingoftissue.PartIof the book concentrates on the preparation of tissue, providing an overview of fixation, embedding, and processing (Chapter 1), and in Chapters 2 and 3, the required techniques for the retrieval of RNA from histological sections. Both routine and specialist histological staining techniques are provided in Part II. These include pro- cols for immuno (Chapters 4–7), lectin (Chapter 8), and hybridization (Chapter 9) histochemistry,histologicalstaining (Chapters10and11),aswellasspecificmethods for the in situ identification of hypoxia (Chapter 12) and apoptosis (Chapter 13). Finally, Part III details advances in imaging (Chapters 14–16) and image analysis (Chapter 17). It is hoped that this volume will provide molecular biologists with the basic his- chemical techniques and histologists with the molecular techniques to realise the potential of their resource. We are indebted to the authors for their generosity in sharing these protocols.

A propos du livre

Présentation de l'éditeur

This book provides molecular biologists with the basic histochemical techniques and histologists with the molecular techniques necessary to realize the potential of their resource. Authoritative and cutting-edge, the book covers a wide range of techniques.

Détails de l'édition

• Éditeur: Humana
• Date d'édition: 2016
• Langue: anglais
• ISBN 10: 1493956949
• ISBN 13: 9781493956944
• Reliure: Broché
• Nombre de pages: 240
• Éditeur: Hewitson Tim D., Darby Ian A.
• Coordonnées du fabricant: GPSR Kontakt; ProductSafety@springernature.com; ; Slevogtstraße 3-5; Heidelberg; 69126; Allemagne