The Inflammasome: Methods and Protocols - Couverture souple

Couverture souple

ISBN 10 : 1493960113 ISBN 13 : 9781493960118

Editeur : Humana, 2016

Synopsis

Inflammasomes have a seemingly simple architecture. A sensor molecule of the NLR or PYHIN protein family recruits the common adapter molecule apoptosis-associated speck-like protein containing a CARD (ASC). Even so, inflammasome activation is highly regulated at multiple steps and precise details of how inflammasomes are activated at the molecular level remain largely unknown. In The Inflammasome: Methods and Protocols, expert researchers in the field detail methods and protocols to study multiple aspects of inflammasome function. These methods include the generation of inflammasome stimuli, perturbance of cellular homeostasis, assessment of ASC speck formation, monitoring of caspase-1 activity and processing, activation of IL-1β cytokines, and analysis of inflammasome assembly. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Détails de l'édition

• Éditeur: Humana
• Date d'édition: 2016
• Langue: anglais
• ISBN 10: 1493960113
• ISBN 13: 9781493960118
• Reliure: Broché
• Nombre de pages: 240
• Éditeur: De Nardo Christine M., Latz Eicke
• Coordonnées du fabricant: ProductSafety@springernature.com; ProductSafety@springernature.com; ; Poststr. 9; Darmstadt; 64293; Allemagne