Biolistic DNA Delivery: Methods and Protocols - Couverture souple

Couverture souple

ISBN 10 : 1493960687 ISBN 13 : 9781493960682

Editeur : Humana, 2016

Synopsis

Biolistic transfection represents a direct physical gene transfer approach in which nucleic acids are precipitated on biologically inert high-density microparticles (usually gold or tungsten) and delivered directly through cell walls and/or membranes into the nucleus of target cells by high-velocity acceleration using a ballistic device such as the gene gun. Biolistic DNA Delivery: Methods and Protocols provides a comprehensive collection of detailed protocols intended to provide the definitive practical guide for the novice as well as for the advanced gene transfer expert on how to introduce nucleic acids into eukaryotic cells using the biolistic technique. Split into six convenient sections, this detailed volume covers biolistic gene transfer into plants, nematodes, and mammalian cells, both in vitro and in vivo, as well as the use of gene gun-mediated DNA vaccination in various experimental animal models of human diseases, and the description of biolistic delivery of molecules other than nucleic acids. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

All-inclusive and cutting-edge, Biolistic DNA Delivery: Methods and Protocols brings together the knowledge and the experience of leading experts in the field of gene transfer in order to serve all researchers who wish to further our abilities in this vital field.

A propos du livre

Présentation de l'éditeur

Another key addition to Springer’s Methods in Molecular Biology series, this volume includes methods for direct biolistic transfection of DNA using ballistic devices such as a gene gun. Lab-ready protocols and expert troubleshooting guarantee reproducibility.

Détails de l'édition

• Éditeur: Humana
• Date d'édition: 2016
• Langue: anglais
• ISBN 10: 1493960687
• ISBN 13: 9781493960682
• Reliure: Broché
• Nombre de pages: 432
• Éditeur: Sudowe Stephan, Reske-Kunz Angelika B.
• Coordonnées du fabricant: ProductSafety@springernature.com; ProductSafety@springernature.com; ; Poststr. 9; Darmstadt; 64293; Allemagne