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ISBN 10 : 1493961004 ISBN 13 : 9781493961009

Editeur : Humana Press Inc., 2016

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The past decade has seen major advances in our understanding of bone biology. This revised and updated second edition includes the step-by-step detail essential for reproducible results and features expert commentary on numerous study techniques.

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A collection of the latest laboratory techniques for the study of bone and bone tissue. Described in step-by-step detail, these readily reproducible methods cover such topics as the isolation and culture of bone cells, the preparation of bone tissue for histological and ultrastructural analysis, methods for the measurement of bone strength and for mechanical studies, and how to use digital imaging techniques in the analysis of bone.

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�diteur: Humana Press Inc.
Date d'�dition: 2016
Langue: anglais
ISBN 10: 1493961004
ISBN 13: 9781493961009
Reliure: Broch�
Nombre de pages: 621
�diteur: Helfrich Miep H., Ralston Stuart H.
Coordonn�es du fabricant: ProductSafety@springernature.com
ProductSafety@springernature.com

Poststr. 9
Darmstadt
64293
Allemagne

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Bone Research Protocols

Stuart H. Ralston

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ISBN 10 : 1493961004 ISBN 13 : 9781493961009

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Taschenbuch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Studies over the past decade have continued to bring tremendous advances to our understanding of bone biology. New pathways have been discovered and expanded our knowledge of the ways in which genes and gene products affect bone cells and thereby bone mass and bone strength. In Bone Research Protocols, Second Edition, expert researchers in the field detail many methods commonly used to study bone biology. Focusing mainly on in vitro methods, this volume gives techniques for isolation, culture and functional analysis of all bone cell types and details a range of imaging methods, including light and ultrastructural microscopy and live cell imaging. Some important in vivo techniques are included, such as analysis of bone resorption and imaging using X rays, fluorescent or luminescent techniques. Methods for study of proteins and nucleic acid are included and methods for analysis of bone composition, measurement of bone strength, and response to mechanical stimulation are described. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Bone Research Protocols, Second Edition seeks to aid scientists in the bone field to establish new techniques in their laboratories. 660 pp. Englisch. N� de r�f. du vendeur 9781493961009

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Bone Research Protocols

Helfrich, Miep H.|Ralston, Stuart H.

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Etat : New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Contains a catalogue of protocols to assist researchers in the pursuit of mechanistic studiesProvides step-by-step detail essential for reproducible resultsContains key notes and implementation advice from the expertsStudies over. N� de r�f. du vendeur 447957067

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Stuart H. Ralston

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Taschenbuch. Etat : Neu. This item is printed on demand - Print on Demand Titel. Neuware -Studies over the past decade have continued to bring tremendous advances to our understanding of bone biology. New pathways have been discovered and expanded our knowledge of the ways in which genes and gene products affect bone cells and thereby bone mass and bone strength. In Bone Research Protocols, Second Edition, expert researchers in the field detail many methods commonly used to study bone biology. Focusing mainly on in vitro methods, this volume gives techniques for isolation, culture and functional analysis of all bone cell types and details a range of imaging methods, including light and ultrastructural microscopy and live cell imaging. Some important in vivo techniques are included, such as analysis of bone resorption and imaging using X rays, fluorescent or luminescent techniques. Methods for study of proteins and nucleic acid are included and methods for analysis of bone composition, measurement of bone strength, and response to mechanical stimulation are described. Written in the highly successful Methods in Molecular Biology� series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Bone Research Protocols, Second Edition seeks to aid scientists in the bone field to establish new techniques in their laboratories.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 660 pp. Englisch. N� de r�f. du vendeur 9781493961009

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Stuart H. Ralston

Edit� par Humana Press, Humana Press, 2016

ISBN 10 : 1493961004 ISBN 13 : 9781493961009

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Taschenbuch. Etat : Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - Studies over the past decade have continued to bring tremendous advances to our understanding of bone biology. New pathways have been discovered and expanded our knowledge of the ways in which genes and gene products affect bone cells and thereby bone mass and bone strength. In Bone Research Protocols, Second Edition, expert researchers in the field detail many methods commonly used to study bone biology. Focusing mainly on in vitro methods, this volume gives techniques for isolation, culture and functional analysis of all bone cell types and details a range of imaging methods, including light and ultrastructural microscopy and live cell imaging. Some important in vivo techniques are included, such as analysis of bone resorption and imaging using X rays, fluorescent or luminescent techniques. Methods for study of proteins and nucleic acid are included and methods for analysis of bone composition, measurement of bone strength, and response to mechanical stimulation are described. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Bone Research Protocols, Second Edition seeks to aid scientists in the bone field to establish new techniques in their laboratories. N� de r�f. du vendeur 9781493961009

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