Mass Spectrometry of Glycoproteins: Methods and Protocols - Couverture souple

Couverture souple

ISBN 10 : 1493962035 ISBN 13 : 9781493962037

Editeur : Humana, 2016

Synopsis

Glycosylation is the most abundant post-translational modification of proteins. Estimates vary widely, but a common assessment is that upwards of 50% of eukaryotic proteins are modified by some type of glycan. In Mass Spectrometry of Glycoproteins: Methods and Protocols, expert researchers in the field detail many of the methods that are now commonly used for glycoproteomics. These methods and techniques include robust sample preparation techniques; advanced chromatographic strategies for improving dynamic range; state-of-the-art mass spectrometry instrumentation and associated ionization and fragmentation methods; and informatics tools used for identifying glycoproteins and characterizing the associated glycans. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Mass Spectrometry of Glycoproteins: Methods and Protocol is an essential resource for those who work at the interface of glycobiology and mass spectrometry.

A propos du livre

Présentation de l'éditeur

This comprehensive new resource in Springer’s Methods in Molecular Biology series features contributions from leading researchers who provide expert advice and reproducible, cutting-edge protocols for examining glycoproteins through mass spectrometry.

Détails de l'édition

• Éditeur: Humana
• Date d'édition: 2016
• Langue: anglais
• ISBN 10: 1493962035
• ISBN 13: 9781493962037
• Reliure: Broché
• Nombre de pages: 352
• Éditeur: Kohler Jennifer J., Patrie Steven M.
• Coordonnées du fabricant: ProductSafety@springernature.com; ProductSafety@springernature.com; ; Poststr. 9; Darmstadt; 64293; Allemagne