Part I: Detection and Quantification of pADPr
1. Quantitation of Poly(ADP-ribose) by Isotope Dilution Mass Spectrometry
Tabea Zubel, Rita Martello, Alexander Bürkle, and Aswin Mangerich
2. Quantification of PARP Activity in Human Tissues: Ex Vivo Assays in Blood Cells and Immunohistochemistry in Human Biopsies
Eszter M. Horvath, Zsuzsanna K. Zsengellér, and Csaba Szabo
3. Detecting and Quantifying pADPr In Vivo
Yi-Chen Lai, Rajesh K. Aneja, Margaret A. Satchell, and Robert S.B. Clark
4. Compartment-Specific Poly-ADP-Ribose Formation as a Biosensor for Subcellular NAD Pools
Magali R. VanLinden, Marc Niere, Andrey A. Nikiforov, Mathias Ziegler, and Christian Dölle
5. Cell Cycle Resolved Measurements of Poly(ADP-ribose) Formation and DNA Damage Signaling by Quantitative Image-Based Cytometry
Jone Michelena and Matthias Altmeyer
Part II: Identification of Protein Targets
6. Detecting Protein ADP-Ribosylation Using a Clickable Aminooxy Probe
Rory K. Morgan and Michael S. Cohen
7. ADP-Ribosylated Peptide Enrichment and Site Identification: The Phosphodiesterase-Based Method
Casey M. Daniels, Shao-En Ong, and Anthony K.L. Leung
8. Using Clickable NAD+ Analogues to Label Substrate Proteins of PARPs
Lu Zhang and Hening Lin
9. Identification of Protein Substrates of Specific PARP Enzymes Using Analog-Sensitive PARP Mutants and a 'Clickable' NAD+ Analog
Bryan A. Gibson and W. Lee Kraus
10. Identification of ADP-Ribose Acceptor Sites on In Vitro Modified Proteins by Liquid Chromatography - Tandem Mass Spectrometry
Mario Leutert, Vera Bilan, Peter Gehrig, and Michael O. Hottiger
11. Proteome-Wide Identification of In Vivo ADP-Ribose Acceptor Sites by Liquid Chromatography -Tandem Mass Spectrometry
Sara C. Larsen, Mario Leutert, Vera Bilan, Rita Martello, Stephanie Jungmichel, Clifford Young, Michael O. Hottiger, and Michael L. Nielsen
Part III: Functional Analysis
12. Poly(ADP-Ribose)-Dependent Chromatin Remodeling in DNA Repair
Théo Lebeaupin, Rebecca Smith, Sébastien Huet, and Gyula Timinszky
13. Methods to Assess the Role of Poly(ADP-Ribose) Polymerases in Regulating Mitochondrial Oxidative Function
Edit Mikó, Tünde Kovács, Tamás Fodor, and Péter Bai
14. Approaches for Investigating Translational Regulation Controlled by PARP1: Biotin-Based UV-Crosslinking and Luciferase Reporter Assay
Yingbiao Ji
15. Methodology to Identify Poly-ADP-Ribose Polymerase 1 (PARP1)
Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.
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Hardcover. Etat : new. Hardcover. This book presents multiple new and classical methods for studying the vital poly-ADP-ribose (pADPr) pathway. Beginning with techniques for the detection and quantification of the product of poly(ADP-ribose) polymerase (PARP) enzymatic activity and detection of variation in pADPr production during the cell cycle, the volume continues with sections on the identification of pADPr protein acceptors, methods focusing on studying molecular mechanisms of PARP functions in eukaryotic cells, particularly those involved in control of DNA repair and oxidative stress, as well as in expression regulation, approaches to the in vitro reconstitution of PARP-1 interaction with chromatin, the development and testing of small molecule PARP inhibitors, and the functions of understudied members of PARP family. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Poly(ADP-Ribose) Polymerase: Methods and Protocols, Second Edition serves as an ideal companion to the first edition for scientists whose investigations involve this important pathway.The chapter 'Identifying and Validating Tankyrase Binders and Substrates: A Candidate Approach' is published open access under a CC BY 4.0 license. Shipping may be from multiple locations in the US or from the UK, depending on stock availability. N° de réf. du vendeur 9781493969920
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Buch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This book presents multiple new and classical methods for studying the vital poly-ADP-ribose (pADPr) pathway. Beginning with techniques for the detection and quantification of the product of poly(ADP-ribose) polymerase (PARP) enzymatic activity and detection of variation in pADPr production during the cell cycle, the volume continues with sections on the identification of pADPr protein acceptors, methods focusing on studying molecular mechanisms of PARP functions in eukaryotic cells, particularly those involved in control of DNA repair and oxidative stress, as well as in expression regulation, approaches to the in vitro reconstitution of PARP-1 interaction with chromatin, the development and testing of small molecule PARP inhibitors, and the functions of understudied members of PARP family. Written for the highly successfulMethods in Molecular Biologyseries, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical,Poly(ADP-Ribose) Polymerase: Methods and Protocols, Second Editionserves as an ideal companion to the first edition for scientists whose investigations involve this important pathway.The chapter 'Identifying and Validating Tankyrase Binders and Substrates: A Candidate Approach' is published open access under a CC BY 4.0 license. 548 pp. Englisch. N° de réf. du vendeur 9781493969920
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Gebunden. Etat : New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Includes updated, cutting-edge methods and protocols for the study of PARP researchProvides step-by-step detail essential for reproducible resultsContains key notes and implementation advice from the expertsThis book presents multi. N° de réf. du vendeur 144311455
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Buch. Etat : Neu. Neuware -This book presents multiple new and classical methods for studying the vital poly-ADP-ribose (pADPr) pathway. Beginning with techniques for the detection and quantification of the product of poly(ADP-ribose) polymerase (PARP) enzymatic activity and detection of variation in pADPr production during the cell cycle, the volume continues with sections on the identification of pADPr protein acceptors, methods focusing on studying molecular mechanisms of PARP functions in eukaryotic cells, particularly those involved in control of DNA repair and oxidative stress, as well as in expression regulation, approaches to the in vitro reconstitution of PARP-1 interaction with chromatin, the development and testing of small molecule PARP inhibitors, and the functions of understudied members of PARP family. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 548 pp. Englisch. N° de réf. du vendeur 9781493969920
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Buch. Etat : Neu. Druck auf Anfrage Neuware - Printed after ordering - This book presents multiple new and classical methods for studying the vital poly-ADP-ribose (pADPr) pathway. Beginning with techniques for the detection and quantification of the product of poly(ADP-ribose) polymerase (PARP) enzymatic activity and detection of variation in pADPr production during the cell cycle, the volume continues with sections on the identification of pADPr protein acceptors, methods focusing on studying molecular mechanisms of PARP functions in eukaryotic cells, particularly those involved in control of DNA repair and oxidative stress, as well as in expression regulation, approaches to the in vitro reconstitution of PARP-1 interaction with chromatin, the development and testing of small molecule PARP inhibitors, and the functions of understudied members of PARP family. Written for the highly successfulMethods in Molecular Biologyseries, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical,Poly(ADP-Ribose) Polymerase: Methods and Protocols, Second Editionserves as an ideal companion to the first edition for scientists whose investigations involve this important pathway.The chapter 'Identifying and Validating Tankyrase Binders and Substrates: A Candidate Approach' is published open access under a CC BY 4.0 license. N° de réf. du vendeur 9781493969920
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