Synopsis
Part I Updated Blood Processing and Handling Strategies
1. Direct Assessment of Plasma/Serum Sample Quality for Proteomics Biomarker Investigation
Viviana Greco, Cristian Piras, Luisa Pieroni, and Andrea Urbani
2. A Protocol for the Preparation of Cryoprecipitate and Cryo-Depleted Plasma
Rosemary L Sparrow, David W Greening, and Richard J Simpson
3. A Proteomic Protocol for the Preparation of Platelet Concentrates
David W Greening, Richard J Simpson, and Rosemary L Sparrow
Part II Updated Fractionation Strategies for In-depth Blood Proteome Analysis
4. Bead-based and Multiplexed Immunoassays for Protein Profiling via Sequential Affinity Capture
Elin Birgersson, Jochen M. Schwenk, and Burcu Ayoglu
5. Affinity Proteomics for Fast, Sensitive, Quantitative Analysis of Proteins in Plasma
John P O'Grady, Kevin W Meyer, and Derrick N Poe
6. Characterization of the Low-molecular Weight Human Plasma Peptidome
David W. Greening and Richard J. Simpson
7. In-depth, Reproducible Analysis of Human Plasma using IgY 14 and SuperMix Immunodepletion
Lynn A. Beer, Bonnie Ky, Kurt T. Barnhart, and David W. Speicher
8. Low-Molecular-Weight Plasma Proteome Analysis Using Top-Down Mass Spectrometry
Dong Huey Cheon, Eun Gyeong Yang, Cheolju Lee, and Ji Eun Lee
9. Identification of Post-Translational Modifications from Serum/Plasma by Immunoaffinity Enrichment and LC-MS/MS Analysis without Depletion of Abundant Proteins
Hongbo Gu, Jianmin Ren, Xiaoying Jia, and Matthew P. Stokes
10. Identification of Core-fucosylated Glycoproteome in Human Plasma
Qichen Cao, Qing Zhao, Xiaohong Qian, and Wantao Ying
Part III Updated Proteome Analysis of Blood Cell Components, Vesicles, and Blood-related Fluids
11. Proteomic Analysis of Blood Extracellular Vesicles in Cardiovascular Disease by LC-MS/MS Analysis
Montserrat Baldan-Martin, Fernando de la Cuesta, Gloria Alvarez-Llamas, Gema Ruiz-Hurtado, Luis M. Ruilope, and Maria G. Barderas
12. Targeted Approach For Proteomic Analysis of a Hidden Membrane Protein
Tania Martins-Marques, Sandra I. Anjo, Teresa Ribeiro-Rodrigues, Bruno Manadas, and Henrique Girao
13. Red Blood Cells in Clinical Proteomics
Ana Sofia Carvalho, Manuel S Rodriguez, and Rune Matthiesen
14. High-throughput Quantitative Lipidomics Analysis of Non-esterified Fatty Acids in Plasma by LC-MS
Nicolas Christinat, Delphine Morin-Rivron, and Mojgan Masoodi
15. Simultaneous Enrichment of Plasma Extracellular Vesicles and Glycoproteome for Studying Disease Biomarkers
Sunil S. Adav and Siu Kwan Sze
16. Lipidomics of Human Blood Plasma by High Resolution Shotgun Mass Spectrometry
Susanne Sales, Oskar Knittelfelder, and Andrej Shevchenko
17. Proteomics Analysis of Circulating Serum Exosomes
Antonius Koller, Purvi Patel, Jenny Kim Kim, and Emily I. Chen
Part IV Antibody-based Discovery and Targeted Proteomics
18. High-density Serum/Plasma Reverse Phase Protein Arrays
Cecilia Hellström, Tea Dodig-Crnkovic, Mun-Gwan Hong, Jochen M. Schwenk, Peter Nilsson, and Ronald Sjöberg
19. Antibody Colocalization Microarray for Cross-reactivity free Multiplexed Protein Analysis
Véronique Laforte, Pik-Shan Lo, Huiyan Li, and David Juncker
20. Surface Profiling of Extracellular Vesicles From Plasma or Ascites Fluid using Dotscan Antibody Microarrays
Larissa Belov, Susannah Hallal, Kieran Matic, Jerry Zhou, Sandra Wissmueller, Nuzhat Ahmed, Sumaiya Tanjil, Stephen P. Mulligan, O. Giles Best, Richard J. Simpson, and Richard I. Christopherson
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Serum/Plasma Proteomics. Methods and Protocols.
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Buch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Over the past decade, there has been an increase in powerful proteomics technologies that allow greater fundamental insights into the blood proteome. Further developments in informatic analyses, software developments, and computational tools are providing insights into large data sets, open-source data along with large-scale application of bioinformatics. Serum/Plasma Proteomics: Methods and Protocols, Second Editionis a comprehensive resource of protocols for areas, pre-analytical through to analytical, of plasma and serum proteomics. Divided into five convenient sections, covering fractionation strategies for in-depth blood proteome analysis, defined procedures for blood collection, handling and storage, detailed protocols for performing both antibody-based and non-antibody based quantitative assays, proteome analysis of blood cell compartments, circulating nanomebraneous vesicles and blood-related fluids, data management, statistical design, and bioinformatic challenges. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Serum/Plasma Proteomics: Methods and Protocols, Second Edition delivers a valuable foundation for the development and application of blood-based proteomics, and further incorporates blood cell components, including platelets, red blood cells, circulating extracellular vesicles/exosomes, and related biofluids. 568 pp. Englisch. N° de réf. du vendeur 9781493970568
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Etat : Neu. 2nd ed. 2017. 551 S. Over the past decade, there has been an increase in powerful proteomics technologies that allow greater fundamental insights into the blood proteome. Further developments in informatic analyses, software developments, and computational tools are providing insights into large data sets, open-source data along with large-scale application of bioinformatics. SerumPlasma Proteomics: Methods and Protocols, Second Edition is a comprehensive resource of protocols for areas, pre-analytical through to analytical, of plasma and serum proteomics. Divided into five convenient sections, covering fractionation strategies for in-depth blood proteome analysis, defined procedures for blood collection, handling and storage, detailed protocols for performing both antibody-based and non-antibody based quantitative assays, proteome analysis of blood cell compartments, circulating nanomebraneous vesicles and blood-related fluids, data management, statistical design, and bioinformatic challenges. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, SerumPlasma Proteomics: Methods and Protocols, Second Edition delivers a valuable foundation for the development and application of blood-based proteomics, and further incorporates blood cell components, including platelets, red blood cells, circulating extracellular vesiclesexosomes, and related biofluids. Sprache: Deutsch Gewicht in Gramm: 2000. N° de réf. du vendeur 480129
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Buch. Etat : Neu. Serum/Plasma Proteomics | Methods and Protocols | Richard J. Simpson (u. a.) | Buch | xvi | Englisch | 2017 | Springer New York | EAN 9781493970568 | Verantwortliche Person für die EU: Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin, juergen[dot]hartmann[at]springer[dot]com | Anbieter: preigu Print on Demand. N° de réf. du vendeur 109512696
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Buch. Etat : Neu. Neuware -Over the past decade, there has been an increase in powerful proteomics technologies that allow greater fundamental insights into the blood proteome. Further developments in informatic analyses, software developments, and computational tools are providing insights into large data sets, open-source data along with large-scale application of bioinformatics. Serum/Plasma Proteomics: Methods and Protocols, Second Edition is a comprehensive resource of protocols for areas, pre-analytical through to analytical, of plasma and serum proteomics. Divided into five convenient sections, covering fractionation strategies for in-depth blood proteome analysis, defined procedures for blood collection, handling and storage, detailed protocols for performing both antibody-based and non-antibody based quantitative assays, proteome analysis of blood cell compartments, circulating nanomebraneous vesicles and blood-related fluids, data management, statistical design, and bioinformatic challenges. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Serum/Plasma Proteomics: Methods and Protocols, Second Edition delivers a valuable foundation for the development and application of blood-based proteomics, and further incorporates blood cell components, including platelets, red blood cells, circulating extracellular vesicles/exosomes, and related biofluids.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 568 pp. Englisch. N° de réf. du vendeur 9781493970568
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