Articles liés à RNA Detection: Methods and Protocols
Synopsis
Preface...
Table of Contents...
Contributing Authors...
1. The Secret Life of RNA: Lessons from Emerging Methodologies
Caroline Medioni and Florence Besse
2. Quantification of 2'-O-Me Residues in RNA using Next-Generation Sequencing (Illumina RiboMethSeq Protocol)
Lilia Ayadi, Yuri Motorin, and Virginie Marchand 3. Identifying the m6A Methylome by Affinity Purification and Sequencing
Phillip J. Hsu and Chuan He 4. PARIS: Psoralen Analysis of RNA Interactions and Structures with High Throughput and Resolution
Zhipeng Lu, Jing Gong, and Qiangfeng Cliff Zhang 5. Axon-TRAP-RiboTag: Affinity-Purification of Translated mRNAs from Neuronal Axons in Mouse In Vivo
Toshiaki Shigeoka, Jane Jung, Christine E. Holt, and Hosung Jung 6. LCM-Seq: A Method for Spatial Transcriptomic Profiling using Laser Capture Microdissection Coupled with PolyA-Based RNA Sequencing
Susanne Nichterwitz, Julio Aguila Benitez, Rein Hoogstraaten, Qiaolin Deng, and Eva Hedlund 7. Spatial Transcriptomics: Constructing a Single-Cell Resolution Transcriptome-Wide Expression Atlas
Kaia Achim, Hernando Martínez Vergara, and Jean-Baptiste Pettit 8. Single mRNA Molecule Detection in Drosophila
Shawn C. Little and Thomas Gregor 9. Detection and Automated Analysis of Single Transcripts at Subcellular Resolution in Zebrafish Embryos
L. Carine Stapel, Coleman Broaddus, and Nadine L. Vastenhouw 10. Super-Resolution Single Molecule FISH at the Drosophila Neuromuscular Junction
Joshua S. Titlow, Lu Yang, Richard M. Parton, Ana Palanca, and Ilan Davis 11. Detection of mRNA and Associated Molecules by ISH-IEM on Frozen Sections
Catherine Rabouille 12. Hybridization Chain Reaction for Direct mRNA Detection without Nucleic Acid Purification
Yao Xu and Zhi Zheng 13. In Situ Detection of MicroRNA Expression with RNAscope Probes
Viravuth P. Yin 14. Padlock Probes to Detect Single Nucleotide Polymorphisms
Tomasz Krzywkowski and Mats Nilsson 15. Quantifying Gene Expression in Living Cells with Ratiometric Bimolecular Beacons
Yantao Yang, Mingming Chen, Christopher K. Krueger, Andrew Tsourkas, and Antony K. Chen 16. Optimizing Molecular Beacons for Intracellular Analysis of RNA
Mingming Chen, Yantao Yang, Christopher K. Krueger, and Antony K. Chen 17. Live Imaging of Nuclear RNPs in Mammalian Complex Tissue with ECHO-liveFISH
Dan Ohtan Wang 18. In Vivo Visualization and Function Probing of Transport mRNPs using Injected FIT Probes
Jasmine Chamiolo, Imre Gaspar, Anne Ephrussi, and Oliver Seitz 19. Visualizing RNA in Live Bacterial Cells using Fluorophore- and Quencher-Binding Aptamers
Murat Sunbul, Ankita Arora, and Andres Jäschke 20. Method for Imaging Live-Cell RNA using an RNA Aptamer and a Fluorescent Probe
Shin-ichi Sato, Kenji Yatsuzuka, Yousuke Katsuda, and Motonari Uesugi 21. RNA Live Imaging in the Model Microorganism Ustilago maydis
S. Zander, K. Müntjes, and M. Feldbrügge 22. Real-Time Fluorescence Imaging of Single-Molecule Endogenous Non-Coding RNA in Living Cells
Hideaki Yoshimura and Takeaki Ozawa 23. Live Imaging of mRNA Synthesis in Drosophila
Hernan G. Garcia and Thomas Gregor 24. Imaging Newly Transcribed RNA in Cells by Using a Clickable Azide-Modified UTP Analog
Anupam A. Sawan
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RNA Detection
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Etat : New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Includes cutting-edge methods and protocolsProvides step-by-step detail essential for reproducible resultsContains key notes and implementation advice from the expertsThis volume introduces different concepts and methods of detec. N° de réf. du vendeur 150705970
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RNA Detection: Methods and Protocols (Methods in Molecular Biology, 1649)
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Buch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume introduces different concepts and methods of detecting RNA in biological material in a variety of model systems. The chapters in this book discuss methods that will answer numerous biological questions that arise in the study of RNAs. Some of the topics covered in this book are single mRNA molecule detection in embryos and neurons ; detection of mRNA and associated molecules by ISH-IEM on frozen sections; optimizing molecular beacons for intracellular analysis of RNA; imaging translation dynamics of single mRNA molecules in live cells; preparation of high-throughput sequencing libraries; and capturing RNA binding proteins in embryos and in cell-culture. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, RNA Detection: Methods and Protocols is a valuable resource for novel and experiences scientist in the expanding field of RNAs. 508 pp. Englisch. N° de réf. du vendeur 9781493972128
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Buch. Etat : Neu. Neuware -This volume introduces different concepts and methods of detecting RNA in biological material in a variety of model systems. The chapters in this book discuss methods that will answer numerous biological questions that arise in the study of RNAs. Some of the topics covered in this book are single mRNA molecule detection in embryos and neurons; detection of mRNA and associated molecules by ISH-IEM on frozen sections; optimizing molecular beacons for intracellular analysis of RNA; imaging translation dynamics of single mRNA molecules in live cells; preparation of high-throughput sequencing libraries; and capturing RNA binding proteins in embryos and in cell-culture. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, RNA Detection: Methods and Protocols is a valuable resource for novel and experiences scientist in the expanding field of RNAs.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 508 pp. Englisch. N° de réf. du vendeur 9781493972128
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RNA Detection : Methods and Protocols
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ISBN 13 : 9781493972128
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Buch. Etat : Neu. Druck auf Anfrage Neuware - Printed after ordering - This volume introduces different concepts and methods of detecting RNA in biological material in a variety of model systems. The chapters in this book discuss methods that will answer numerous biological questions that arise in the study of RNAs. Some of the topics covered in this book are single mRNA molecule detection in embryos and neurons ; detection of mRNA and associated molecules by ISH-IEM on frozen sections; optimizing molecular beacons for intracellular analysis of RNA; imaging translation dynamics of single mRNA molecules in live cells; preparation of high-throughput sequencing libraries; and capturing RNA binding proteins in embryos and in cell-culture. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, RNA Detection: Methods and Protocols is a valuable resource for novel and experiences scientist in the expanding field of RNAs. N° de réf. du vendeur 9781493972128
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