Part I: Detection and Quantification of Quorum Sensing Signal Molecules
1. Use of Whole Cell Bioassays for Screening Quorum Signaling, Quorum-Interference, and Biofilm Dispersion
Starla G. Thornhill and Robert J.C. McLean
2. Detection of 2-Alkyl-4-Quinolones Using Biosensors
Matthew P. Fletcher, Stephen P. Diggle, Miguel Cámara, and Paul Williams
3. "Hot Stuff" The Many Uses of a Radiolabel Assay in Detecting Acyl-Homoserine Lactone Quorum Sensing Signals
Amy L. Schaefer, Caroline S. Harwood, and E. Peter Greenberg
4. Liquid Chromatography/Mass Spectrometry (LC/MS) for the Detection and Quantification of -Acyl-L-Homoserine Lactones (AHLs) and 4-Hydroxy-2-Alkylquinolines (HAQs)
François Lépine, Sylvain Milot, Marie-Christine Groleau, and Eric Déziel
5. Detection of the Bacterial Quorum Sensing Signaling Molecules N-Acyl Homoserine Lactones (HSL) and N-Acyl-Homoserine (HS) with an Enzyme-Linked Immunosorbent Assay (ELISA) and via Ultrahigh Performance Liquid Chromatography Coupled to Mass Spectrometry (UHPLC-MS)
Michael Rothballer, Jenny Uhl, Josie Kunze, Philippe Schmitt-Kopplin, and Anton Hartmann
6. Biosensors for the Detection and Quantification of AI-2 Class Quorum Sensing Compounds
Sathish Rajamani and Richard Sayre
7. Detection of Agr-Type Autoinducing Peptides Produced by Staphylococcus aureus
Ewan J. Murray and Paul Williams
8. Ultra Performance Liquid Chromatography/Mass Spectrometry for the Detection and Quantification of Diffusible Signal Factor (DSF)-Family Quorum Sensing Signals
Lian Zhou, Shuang Sun, Wei Zhang, and Ya-Wen He
9. Rapid Electrochemical Detection of Pseudomonas aeruginosa Signaling Molecules by Boron-Doped Diamond Electrode
Alyah Buzid, John H.T. Luong, F. Jerry Reen, Fergal O'Gara, Jeremy D. Glennon, and Gerard P. McGlacken
10. Detection and Quantification of Butyrolactones from Streptomyces
Marc Biarnes-Carrera, Rainer Breitling, and Eriko Takano
Part II: Methods for the Studying of Quorum Sensing at the Molecular, Physiological, and Population Level
11. Fluorescence Quenching Studies of γ-Butyrolactone Binding Protein (CprB) from Streptomyces coelicolor A3(2)
Jessy Mariam and Ruchi Anand
12. Methods to Study Solo/Orphan Quorum Sensing Receptors
Vittorio Venturi, Sujatha Subramoni, Anice Sabag-Daigle, and Brian M.M. Ahmer
13. Enzymatic Assays to Investigate Acyl-Homoserine Lactone Autoinducer Synthases
Daniel Shin and Rajesh Nagarajan
14. Global Expr
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Gebunden. Etat : New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Includes cutting-edge techniques for the study of quorum sensingProvides step-by-step detail essential for reproducible resultsContains key implementation advice from the expertsThis detailed volume provides scientists interested. N° de réf. du vendeur 150560968
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Buch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed volume provides scientists interested in quorum sensing with a broad spectrum of methods and protocols useful for studying bacterial communication processes at the chemico-physical, molecular, and physiological level. Divided into three sections, the content covers detection and quantification of quorum sensing signal molecules, methods for the studying of quorum sensing at the molecular, physiological, and population level, as well as identification and characterization of anti-quorum sensing agents. Written for the highly successfulMethods in Molecular Biologyseries, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge,Quorum Sensing: Methods and Protocolsserves as a comprehensive guide to the most important methodology currently available in the field. 396 pp. Englisch. N° de réf. du vendeur 9781493973088
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