Quorum Sensing: Methods and Protocols - Couverture rigide

Couverture rigide

ISBN 10 : 1493973088 ISBN 13 : 9781493973088

Editeur : Humana Press Inc., 2017

Synopsis

Part I: Detection and Quantification of Quorum Sensing Signal Molecules

1. Use of Whole Cell Bioassays for Screening Quorum Signaling, Quorum-Interference, and Biofilm Dispersion

Starla G. Thornhill and Robert J.C. McLean

2. Detection of 2-Alkyl-4-Quinolones Using Biosensors

Matthew P. Fletcher, Stephen P. Diggle, Miguel Cámara, and Paul Williams

3. "Hot Stuff" The Many Uses of a Radiolabel Assay in Detecting Acyl-Homoserine Lactone Quorum Sensing Signals

Amy L. Schaefer, Caroline S. Harwood, and E. Peter Greenberg

4. Liquid Chromatography/Mass Spectrometry (LC/MS) for the Detection and Quantification of -Acyl-L-Homoserine Lactones (AHLs) and 4-Hydroxy-2-Alkylquinolines (HAQs)

François Lépine, Sylvain Milot, Marie-Christine Groleau, and Eric Déziel

5. Detection of the Bacterial Quorum Sensing Signaling Molecules N-Acyl Homoserine Lactones (HSL) and N-Acyl-Homoserine (HS) with an Enzyme-Linked Immunosorbent Assay (ELISA) and via Ultrahigh Performance Liquid Chromatography Coupled to Mass Spectrometry (UHPLC-MS)

Michael Rothballer, Jenny Uhl, Josie Kunze, Philippe Schmitt-Kopplin, and Anton Hartmann

6. Biosensors for the Detection and Quantification of AI-2 Class Quorum Sensing Compounds

Sathish Rajamani and Richard Sayre

7. Detection of Agr-Type Autoinducing Peptides Produced by Staphylococcus aureus

Ewan J. Murray and Paul Williams

8. Ultra Performance Liquid Chromatography/Mass Spectrometry for the Detection and Quantification of Diffusible Signal Factor (DSF)-Family Quorum Sensing Signals

Lian Zhou, Shuang Sun, Wei Zhang, and Ya-Wen He

9. Rapid Electrochemical Detection of Pseudomonas aeruginosa Signaling Molecules by Boron-Doped Diamond Electrode

Alyah Buzid, John H.T. Luong, F. Jerry Reen, Fergal O'Gara, Jeremy D. Glennon, and Gerard P. McGlacken

10. Detection and Quantification of Butyrolactones from Streptomyces

Marc Biarnes-Carrera, Rainer Breitling, and Eriko Takano

Part II: Methods for the Studying of Quorum Sensing at the Molecular, Physiological, and Population Level

11. Fluorescence Quenching Studies of γ-Butyrolactone Binding Protein (CprB) from Streptomyces coelicolor A3(2)

Jessy Mariam and Ruchi Anand

12. Methods to Study Solo/Orphan Quorum Sensing Receptors

Vittorio Venturi, Sujatha Subramoni, Anice Sabag-Daigle, and Brian M.M. Ahmer

13. Enzymatic Assays to Investigate Acyl-Homoserine Lactone Autoinducer Synthases

Daniel Shin and Rajesh Nagarajan

14. Global Expr

A propos du livre

À propos de la quatrième de couverture

This detailed volume provides scientists interested in quorum sensing with a broad spectrum of methods and protocols useful for studying bacterial communication processes at the chemico-physical, molecular, and physiological level. Divided into three sections, the content covers detection and quantification of quorum sensing signal molecules, methods for the studying of quorum sensing at the molecular, physiological, and population level, as well as identification and characterization of anti-quorum sensing agents. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Quorum Sensing: Methods and Protocols serves as a comprehensive guide to the most important methodology currently available in the field.

Détails de l'édition

• Éditeur: Humana Press Inc.
• Date d'édition: 2017
• Langue: anglais
• ISBN 10: 1493973088
• ISBN 13: 9781493973088
• Reliure: Relié
• Numéro d'édition: 1
• Nombre de pages: 375
• Éditeur: Leoni Livia, Rampioni Giordano
• Coordonnées du fabricant: Springer Nature Customer Service Center GmbH; ProductSafety@springernature.com; ; Europaplatz 3; Heidelberg; 69115; Allemagne