The Surfaceome: Methods and Protocols - Couverture rigide

Couverture rigide

ISBN 10 : 149397551X ISBN 13 : 9781493975518

Editeur : Humana Press Inc., 2017

Synopsis

This volume provides readers with the latest techniques and tools to assess modifications and functions of the surfaceome. The chapters in this book are divided into 4 sections: discovery-based approaches to surfaceome content; targeted approaches for surfaceome content; cell-based function analyses related to surfaceome content; and computational approaches in surfaceome studies. Section 1 focuses on discovery-based approaches for cataloging surfaceome content that analyses the surfaceome of bacteria, avian embryos, and mammalian systems. Section 2 discusses methods that over-express specific targets in Sf9 cells and generate bi-specific antibodies for targeting cancer and somatic cells. Section 3 explores voltage dependent sodium channels and high-content electrophysiological analyses. The final section looks at the new web-based platform known as targets-search. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and thorough, Surfaceome: Methods and Protocols assists in the study of cell surface protein biology and function. It is a valuable resource for all researchers interested in this field.

Détails de l'édition

• Éditeur: Humana Press Inc.
• Date d'édition: 2017
• Langue: anglais
• ISBN 10: 149397551X
• ISBN 13: 9781493975518
• Reliure: Relié
• Numéro d'édition: 1
• Nombre de pages: 360
• Éditeur: Boheler Kenneth R., Gundry Rebekah L.
• Coordonnées du fabricant: ProductSafety@springernature.com; ProductSafety@springernature.com; ; Poststr. 9; Darmstadt; 64293; Allemagne