Sertoli Cells: Methods and Protocols - Couverture rigide

Couverture rigide

ISBN 10 : 1493976974 ISBN 13 : 9781493976973

Editeur : Humana Press Inc., 2018

Synopsis

1. Establishment of Primary Culture of Sertoli Cells

Raquel L. Bernardino, Marco G. Alves, and Pedro F. Oliveira

2. Evaluation of the Purity of Sertoli Cell Primary Cultures

Raquel L. Bernardino, Marco G. Alves, and Pedro F. Oliveira

3. Preparation of Testicular Samples for Histology and Immunohistochemistry

Barbara Bilinska, Anna Hejmej, and Malgorzata Kotula-Balak

4. Rabbit Sertoli Cells: Immunohistochemical Profile from Neonatal to Adult Age

Valeria Grieco and Barbara Banco

5. Identification of Proliferative and Apoptotic Sertoli Cells Using Fluorescence and Confocal Microscopy

Jesús Martínez-Hernández, Vicente Seco-Rovira, Ester Beltrán-Frutos, Victor Quesada-Cubo, Concepción Ferrer, and Luis Miguel Pastor

6. Sertoli Cells Preparation for Co-Immunoprecipitation

Maria João Freitas and Margarida Fardilha

7. Profiling Signaling Proteins in Sertoli Cells by Co-Immunoprecipitation

Maria João Freitas and Margarida Fardilha

8. Phagocytosis by Sertoli Cells: Analysis of Main Phagocytosis Steps by Confocal and Electron Microscopy

Marina G. Yefimova, Nadia Messaddeq, Annie-Claire Meunier, Anne Cantereau, Bernard Jegou, and Nicolas Bourmeyster

9. A Method for In Vivo Induction and Ultrastructural Detection of Mitophagy in Sertoli Cells

Nabil Eid, Yuko Ito, Akio Horibe, Hitomi Hamaoka, and Yoichi Kondo

10. Assessing Autophagy in Sertoli Cells

Chao Liu, Jehangir Khan, and Wei Li

11. Molecular Mechanisms and Signaling Pathways Involved in the Nutritional Support of Spermatogenesis by Sertoli Cells

Luís Crisóstomo, Marco G. Alves, Agostina Gorga, Mário Sousa, María F. Riera, María N. Galardo, Silvina B. Meroni, and Pedro F. Oliveira

12. Assessing Sertoli Cells Metabolic Activity

Ivana Jarak, Pedro F. Oliveira, Gustavo Rindone, Rui Carvalho, María Noel Galardo, María Fernanda Riera, Silvina Meroni, and Marco G. Alves

13. Proteome Profiling of Sertoli Cells Using a GeLC-MS/MS Strategy

Rita Ferreira, Fábio Trindade, and Rui Vitorino

14. Gene Silencing of Human Sertoli Cells Utilizing Small Interfering RNAs

Hong Wang, Qingqing Yuan, Minghui Niu, Liping Wen, Hongyong Fu, Fan Zhou, Weihui Zhang, and Zuping He

15. Testicular Cell Selective Ablation Using Diphtheria Toxin Receptor Transgenic Mice

Diane Rebourcet, Annalucia Darbey, Michael Curley, Peter O'Shaughnessy, and Lee B. Smith

16. Regulation of Blood-Testis Barrier (BTB) Dynamics, Role of the Actin, an

Détails de l'édition

• Éditeur: Humana Press Inc.
• Date d'édition: 2018
• Langue: anglais
• ISBN 10: 1493976974
• ISBN 13: 9781493976973
• Reliure: Relié
• Numéro d'édition: 1
• Nombre de pages: 310
• Éditeur: Alves Marco G., Oliveira Pedro F.
• Coordonnées du fabricant: ProductSafety@springernature.com; ProductSafety@springernature.com; ; Poststr. 9; Darmstadt; 64293; Allemagne