Epigenome Editing: Methods and Protocols - Couverture rigide

9781493977734: Epigenome Editing: Methods and Protocols

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ISBN 10 : 1493977733 ISBN 13 : 9781493977734

Editeur : Humana Press Inc., 2018

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Part I: General Reviews

1. Editing the Epigenome: Overview, Open Questions, and Directions of Future Development

Marianne G. Rots and Albert Jeltsch

2. Zinc Fingers, TALEs and CRISPR Systems: A Comparison of Tools for Epigenome Editing

Charlene Babra Waryah, Colette Moses, Mahira Arooj, and Pilar Blancafort

3. Designing Epigenome Editors: Considerations of Biochemical and Locus Specificities

Dilara Sen and Albert J. Keung

4. Generation of TALE-Based Designer Epigenome Modifiers

Sandra Nitsch and Claudio Mussolino

Part II: Topical Reviews

5. Neuroepigenetic Editing

Peter J. Hamilton, Carissa J. Lim, Eric J. Nestler, and Elizabeth A. Heller

6. Allele-Specific Epigenome Editing

Pavel Bashtrykov and Albert Jeltsch

7. Key to Delivery: The (Epi-)Genome Editing Vector Toolbox

Sabrina Just and Hildegard B�ning

8. CRISPR/dCas9 Switch Systems for Temporal Transcriptional Control

Rutger A.F. Gjaltema and Edda G. Schulz

Part III: Delivery Protocols

9. Delivery of Designer Epigenome Modifiers into Primary Human T Cells

Tafadzwa Mlambo, Marianna Romito, Tatjana I. Cornu, and Claudio Mussolino

10. Viral Expression of Epigenome Editing Tools in Rodent Brain Using Stereotaxic Surgery Techniques

Peter J. Hamilton, Carissa J. Lim, Eric J. Nestler, and Elizabeth A. Heller

11. Stable Expression of Epigenome Editors Via Viral Delivery and Genomic Integration

Carolin Kroll and Philipp Rathert

12. Purified-Protein Delivery to Activate an Epigenetically-Silenced Allele in Mouse Brain

Benjamin Pyles, Barbara J. Bailus, Henriette O'Geen, and David J. Segal

13. Non-Viral Methodology for Efficient Co-Transfection

Jessica A. Kretzmann, Cameron W. Evans, Marck Norret, Pilar Blancafort, and K. Swaminathan Iyer

Part IV: Histone Editing Readout Protocols

14. Chromatin Immunoprecipitation in Human and Yeast Cells

Jessica B. Lee and Albert J. Keung

15. Chromatin Immunoprecipitation and High Throughput Sequencing (ChIP-Seq): Tips and Tricks Regarding the Laboratory Protocol and Initial Downstream Data Analysis

Darren K. Patten, Giacomo Corleone, and Luca Magnani

Part V: DNA Methylation Editing Readout Protocols

16. Generation of Whole Genome Bisulfite Sequencing Libraries for Comprehensive DNA Methylome Analysis

Dulce B. Vargas-Landin, Jahnvi Pfl�ger, and Ryan Lister

17. Approaches for the Analysis and Interpretation of Whole Genome Bisulfite Sequencing Data

Tim Stuart, Sam Buckberry, and Ryan Lister

18. Whole Genome Bisulfite Sequencing for the Analysis of Genome-Wide DNA Methylation and Hydroxymethylation Patterns at Single-Nucleotide Resolution

Magali Kernaleguen, Christian Daviaud, Yimin Shen, Eric Bonnet, Victor Renault, Jean-Fran�ois Deleuze, Florence Mauger, and J�rg Tost

19. Locus-Specific DNA Methylation Analysis by Targeted Deep Bisulfite Sequencing

Elsa Leit�o, Jasmin Beygo, Michael Zeschnigk, Ludger Klein-Hitpass, Marcel Bargull, Sven Rahmann, and Bernhard Horsthemke

20. DNA Methylation Analysis by Bisulfite Conversion Coupled to Double Multipl

Les informations fournies dans la section � Synopsis � peuvent faire r�f�rence � une autre �dition de ce titre.

�diteurHumana Press Inc.
Date d'�dition2018
ISBN 10 1493977733
ISBN 13 9781493977734
ReliureReli�
Langueanglais
Num�ro d'�dition1
Nombre de pages482
�diteurJeltsch Albert, Rots Marianne G.
Coordonn�es du fabricantnon disponible

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Epigenome Editing. Methods and Protocols.

Jeltsch, A. et al (Eds.)

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ISBN 10 : 1493977733 ISBN 13 : 9781493977734

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xiv, 482 p. Hardcover. Versand aus Deutschland / We dispatch from Germany via Air Mail. Einband besto�en, daher M�ngelexemplar gestempelt, sonst sehr guter Zustand. Imperfect copy due to slightly bumped cover, apart from this in very good condition. Stamped. Sprache: Englisch. N� de r�f. du vendeur 5005BB

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Epigenome Editing

Jeltsch, Albert|Rots, Marianne G.

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Etat : New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Includes cutting-edge techniques for the study of epigenome editingProvides step-by-step detail essential for reproducible resultsContains key implementation advice from the expertsIncludes cutting-edge techniques for . N� de r�f. du vendeur 447957518

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Epigenome Editing

Marianne G. Rots

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ISBN 10 : 1493977733 ISBN 13 : 9781493977734

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Buch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed book explores the concepts and applications of epigenome editing, as presented by leading scientists in the field. Beginning with some general and topical reviews, the collection continues by covering the design of DNA-binding devices, optimization of the effector domains, readout of epigenome marks, and approaches for delivery at the cellular and organismal level. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Epigenome Editing: Methods and Protocols will be of great assistance to people new to the field but also to those already engaged, as epigenetic editing is still a relatively unexplored field with many issues to be resolved. 496 pp. Englisch. N� de r�f. du vendeur 9781493977734

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Epigenome Editing

Marianne G. Rots

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ISBN 10 : 1493977733 ISBN 13 : 9781493977734

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Buch. Etat : Neu. Neuware -This detailed book explores the concepts and applications of epigenome editing, as presented by leading scientists in the field. Beginning with some general and topical reviews, the collection continues by covering the design of DNA-binding devices, optimization of the effector domains, readout of epigenome marks, and approaches for delivery at the cellular and organismal level. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 496 pp. Englisch. N� de r�f. du vendeur 9781493977734

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Epigenome Editing : Methods and Protocols

Marianne G. Rots

Edit� par Springer New York, 2018

ISBN 10 : 1493977733 ISBN 13 : 9781493977734

Neuf Couverture rigide

Vendeur : AHA-BUCH GmbH, Einbeck, Allemagne

�valuation du vendeur 5 sur 5 �toiles

Buch. Etat : Neu. Druck auf Anfrage Neuware - Printed after ordering - This detailed book explores the concepts and applications of epigenome editing, as presented by leading scientists in the field. Beginning with some general and topical reviews, the collection continues by covering the design of DNA-binding devices, optimization of the effector domains, readout of epigenome marks, and approaches for delivery at the cellular and organismal level. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Epigenome Editing: Methods and Protocols will be of great assistance to people new to the field but also to those already engaged, as epigenetic editing is still a relatively unexplored field with many issues to be resolved. N� de r�f. du vendeur 9781493977734

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