Skeletal Muscle Regeneration in the Mouse: Methods and Protocols - Couverture souple

9781493981458: Skeletal Muscle Regeneration in the Mouse: Methods and Protocols

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ISBN 10 : 1493981455 ISBN 13 : 9781493981458

Editeur : Springer, 2018

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This volume focuses on the cell biology and physiology of skeletal muscle regeneration. This Book is a collection of classic and cutting edge protocols optimized for mice, but in most cases adaptable to rat or other mammalian models, that will allow an investigator to develop and implement a research study on skeletal muscle regeneration. Chapters address the three major areas of study: provoking regeneration by inducing damage to muscle, analyzing the progenitor cells of skeletal muscle, and quantifying overall muscle function. Subjects discussed include: inducing skeletal muscle injury by eccentric contraction; volumetric muscle loss; single myofiber isolation and culture; satellite cell transplantation; muscle clearing for whole mount immunostaining; luciferase tracking of muscle stem cells; mitochondrial and mitophagy flux analysis; in vivo assessment of muscle contractility; force measurements on single isolated myofibers; and analysis of aerobic respiration in intact skeletal muscle tissue by microplate respirometry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to each respective topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and practical, Skeletal Muscle Regeneration in the Mouse: Methods and Protocols is an essential laboratory reference for research in skeletal muscle growth, damage, repair, degeneration, and regenerative therapy in the mouse model system.

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� propos de l?auteur

Michael Kyba, Ph.D. Associate Professor and Lillehei Endowed Scholar, Lillehei Heart Institute NHH4.126, University of Minnesota, SE Minneapolis, MN 55455, USA

Les informations fournies dans la section � A propos du livre � peuvent faire r�f�rence � une autre �dition de ce titre.

�diteur: Springer
Date d'�dition: 2018
Langue: anglais
ISBN 10: 1493981455
ISBN 13: 9781493981458
Reliure: Broch�
Nombre de pages: 360
�diteur: Kyba Michael
Coordonn�es du fabricant: ProductSafety@springernature.com
ProductSafety@springernature.com

Poststr. 9
Darmstadt
64293
Allemagne

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9781493938087: Skeletal Muscle Regeneration in the Mouse: Methods and Protocols

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ISBN 10 : 1493938088 ISBN 13 : 9781493938087
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Skeletal Muscle Regeneration in the Mouse: Methods and Protocols (eng)

Kyba, Michael

Edit� par Springer, 2018

ISBN 10 : 1493981455 ISBN 13 : 9781493981458

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Skeletal Muscle Regeneration in the Mouse

Michael Kyba

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Taschenbuch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume focuseson the cell biology and physiology of skeletal muscle regeneration. This Bookis a collection of classic and cutting edge protocols optimized for mice, butin most cases adaptable to rat or other mammalian models, that will allow aninvestigator to develop and implement a research study on skeletal muscleregeneration. Chapters address the three major areas of study: provoking regeneration by inducing damage to muscle, analyzing theprogenitor cells of skeletal muscle, and quantifying overall muscle function. Subjects discussed include: inducing skeletal muscle injury byeccentric contraction; volumetric muscle loss; single myofiber isolation andculture; satellite cell transplantation; muscle clearing for whole mountimmunostaining; luciferase tracking of muscle stem cells;mitochondrialand mitophagy flux analysis;in vivo assessment of muscle contractility;force measurements on single isolated myofibers; and analysis of aerobicrespiration in intact skeletal muscle tissue by microplate respirometry.Written in the highlysuccessfulMethods in Molecular Biologyseries format,chapters include introductions to each respective topic, lists of the necessarymaterials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.Cutting edge andpractical, Skeletal Muscle Regeneration in the Mouse: Methods and Protocols is an essential laboratory reference for research inskeletal muscle growth, damage, repair, degeneration, and regenerative therapyin the mouse model system. 360 pp. Englisch. N� de r�f. du vendeur 9781493981458

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Skeletal Muscle Regeneration in the Mouse: Methods and Protocols (Methods in Molecular Biology, 1460)

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Skeletal Muscle Regeneration in the Mouse

Kyba, Michael

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Etat : New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Includes cutting-edge methods and protocols&nbspProvides step-by-step details essential for reproducible results&nbspContains key notes and implementation advice from the expertsMichael Kyba, Ph.D.As. N� de r�f. du vendeur 447957713

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Skeletal Muscle Regeneration in the Mouse | Methods and Protocols

Michael Kyba

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Michael Kyba

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Taschenbuch. Etat : Neu. This item is printed on demand - Print on Demand Titel. Neuware -This volume focuseson the cell biology and physiology of skeletal muscle regeneration. This Bookis a collection of classic and cutting edge protocols optimized for mice, butin most cases adaptable to rat or other mammalian models, that will allow aninvestigator to develop and implement a research study on skeletal muscleregeneration. Chapters address the three major areas of study: provoking regeneration by inducing damage to muscle, analyzing theprogenitor cells of skeletal muscle, and quantifying overall muscle function. Subjects discussed include: inducing skeletal muscle injury byeccentric contraction; volumetric muscle loss; single myofiber isolation andculture; satellite cell transplantation; muscle clearing for whole mountimmunostaining; luciferase tracking of muscle stem cells; mitochondrialand mitophagy flux analysis; in vivo assessment of muscle contractility;force measurements on single isolated myofibers; and analysis of aerobicrespiration in intact skeletal muscle tissue by microplate respirometry. Written in the highlysuccessful Methods in Molecular Biology series formatchapters include introductions to each respective topic, lists of the necessarymaterials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.Cutting edge andpractical, Skeletal Muscle Regeneration in the Mouse: Methods and Protocols is an essential laboratory reference for research inskeletal muscle growth, damage, repair, degeneration, and regenerative therapyin the mouse model system.Springer-Verlag KG, Sachsenplatz 4-6, 1201 Wien 360 pp. Englisch. N� de r�f. du vendeur 9781493981458

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Skeletal Muscle Regeneration in the Mouse : Methods and Protocols

Michael Kyba

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ISBN 10 : 1493981455 ISBN 13 : 9781493981458

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Taschenbuch. Etat : Neu. Druck auf Anfrage Neuware - Printed after ordering - This volume focuseson the cell biology and physiology of skeletal muscle regeneration. This Bookis a collection of classic and cutting edge protocols optimized for mice, butin most cases adaptable to rat or other mammalian models, that will allow aninvestigator to develop and implement a research study on skeletal muscleregeneration. Chapters address the three major areas of study: provoking regeneration by inducing damage to muscle, analyzing theprogenitor cells of skeletal muscle, and quantifying overall muscle function. Subjects discussed include: inducing skeletal muscle injury byeccentric contraction; volumetric muscle loss; single myofiber isolation andculture; satellite cell transplantation; muscle clearing for whole mountimmunostaining; luciferase tracking of muscle stem cells;mitochondrialand mitophagy flux analysis;in vivo assessment of muscle contractility;force measurements on single isolated myofibers; and analysis of aerobicrespiration in intact skeletal muscle tissue by microplate respirometry.Written in the highlysuccessfulMethods in Molecular Biologyseries format,chapters include introductions to each respective topic, lists of the necessarymaterials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.Cutting edge andpractical, Skeletal Muscle Regeneration in the Mouse: Methods and Protocols is an essential laboratory reference for research inskeletal muscle growth, damage, repair, degeneration, and regenerative therapyin the mouse model system. N� de r�f. du vendeur 9781493981458

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