Synopsis
1. Visualizing Wnt Palmiteoylation in Single Cells
Xinxin Gao and Rami N. Hannoush
2. Monitoring Wnt Protein Acylation Using an In Vitro Cyclo-Addition Reaction
Rubina Tuladhar, Nageswari Yarravarapu, and Lawrence Lum
3. Biochemical Methods to Analyze Wnt Protein Secretion
Kathrin Glaeser, Michael Boutros, and Julia Christina Gross
4. Methods for Studying Wnt Protein Modifications/Inactivations by Extracellular Enzymes, Tiki and Notum
Xinjun Zhang and Xi He
5. Probing Wnt Receptor Turnover: A Critical Regulatory Point of Wnt Pathway
Xiaomo Jiang and Feng Cong
6. A Simple Method to Assess Abundance of the b-Catenin Signaling Pool in Cells
Annette S. Flozak, Anna P. Lam, and Cara J. Gottardi
7. Wnt-Dependent Control of Cell Polarity in Cultured Cells
Kristin B. Runkle and Eric S. Witze
8. The Use of Chick Embryos to Study Wnt Signaling/Gradients
Lisa M. Galli, Tiffany Barnes, and Laura W. Burrus9. Monitoring Wnt Signaling in Zebrafish Using Fluorescent Biosensors
Nicola Facchinello, Marco Schiavone, Andrea Vettori, Francesco Argenton, and Natascia Tiso
10. Biochemical Analysis of Tankyrase Activity in Zebrafish In Vitro and In Vivo
Jesung Moon and James F. Amatruda
11. Reconstitution of the Cytoplasmic Regulation of the Wnt Signaling Pathway Using Xenopus Egg Extracts
Annastasia Simone Hyde, Brian I. Hang, and Ethan Lee
12. Delivery of the Porcupine Inhibitor WNT974 in Mice
Li-shu Zhang and Lawrence Lum
13. Use of Primary Calvarial Osteoblasts to Evaluate the Function of Wnt Signaling in Osteogenesis
Zhendong A. Zhong, Nicole J. Ethen, and Bart O. Williams
14. Monitoring Wnt/b-Catenin Signaling in Skin
Amy Ku, Qi Miao, and Hoang Nguyen
15. The Generation of Organoids for Studying Wnt Signaling
Jarno Drost, Benedetta Artegiani, and Hans Clevers16. Methods to Manipulate and Monitor Wnt Signaling in Human Pluripotent Stem Cells
Ian Huggins, David Brafman, and Karl Willert
17. Directed Endothelial Progenitor Differentiation from Human Pluripotent Stem Cells Via Wnt Activation Under Defined Conditions
Xiaoping Bao, Xiaojun Lian, and Sean P. Palecek
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Wnt Signaling
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Taschenbuch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Now poised to evaluate the potential in modulating Wnt signaling for therapeutic agendas in cancer, wound healing, and degenerative disease, this book collects protocols from an exciting area of study. The methodologies include using Wnt modulating chemicals in engineering tissues from induced pluripotent and embryonic stem cells, monitoring Wnt transcriptional responses in diverse tissues such as bone and skin, and using specific biochemical markers of Wnt signaling to either screen molecular libraries or evaluate novel reagents. These protocols also leverage unique experimental strengths from five different model organisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Practical and authoritative, Wnt Signaling: Methods and Protocols aims to support researchers in understanding Wnt signal transduction in any effort to improve outcomes in regenerative medicine and cancer management. 212 pp. Englisch. N° de réf. du vendeur 9781493981854
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Paperback. Etat : new. Paperback. Now poised to evaluate the potential in modulating Wnt signaling for therapeutic agendas in cancer, wound healing, and degenerative disease, this book collects protocols from an exciting area of study. The methodologies include using Wnt modulating chemicals in engineering tissues from induced pluripotent and embryonic stem cells, monitoring Wnt transcriptional responses in diverse tissues such as bone and skin, and using specific biochemical markers of Wnt signaling to either screen molecular libraries or evaluate novel reagents. These protocols also leverage unique experimental strengths from five different model organisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Wnt Signaling: Methods and Protocols aims to support researchers in understanding Wnt signal transduction in any effort to improve outcomes in regenerative medicine and cancer management. Now poised to evaluate the potential in modulating Wnt signaling for therapeutic agendas in cancer, wound healing, and degenerative disease, this book collects protocols from an exciting area of study. Shipping may be from multiple locations in the US or from the UK, depending on stock availability. N° de réf. du vendeur 9781493981854
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