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9781493982431: Cardiac Gene Therapy: Methods and Protocols

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ISBN 10 : 1493982435 ISBN 13 : 9781493982431

Editeur : Humana, 2018

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Part I: Overview

1. Current Methods in Cardiac Gene Therapy: Overview

Kiyotake Ishikawa and Roger J. Hajjar

Part II: Genetic Modification

2. Silencing Genes in the Heart

Henry Fechner, Roland Vetter, Jens Kurreck, and Wolfgang Poller

3. Generation of Efficient miRNA Inhibitors Using Tough Decoy Constructs

Jimeen Yoo, Roger J. Hajjar, and Dongtak Jeong

4. Efficient Genome Editing in Induced Pluripotent Stem Cells with Engineered Nucleases In Vitro

Vittavat Termglinchan, Timon Seeger, Caressa Chen, Joseph C. Wu, and Ioannis Karakikes

5. Direct Cardiac Reprogramming as a Novel Therapeutic Strategy for Treatment of Myocardial Infarction

Hong Ma, Li Wang, Jiandong Liu, and Li Qian

Part III: Gene Delivery Vectors

6. Production and Characterization of Vectors Based on the Cardiotropic AAV Serotype 9

Erik Kohlbrenner and Thomas Weber

7. Cell-Based Measurement of Neutralizing Antibodies Against Adeno-Associated Virus (AAV)

Andreas Jungmann, Oliver M�ller, and Kleopatra Rapti

8. Synthesis of Modified mRNA for Myocardial Delivery

Jason Kondrat, Nishat Sultana, and Lior Zangi

9. Exosomes-Based Gene Therapy for MicroRNA Delivery

Prabhu Mathiyalagan and Susmita Sahoo

10. Lipidoid mRNA Nanoparticles for Myocardial Delivery in Rodents

Irene C. Turnbull, Ahmed A. Eltoukhy, Daniel G. Anderson, and Kevin D. Costa

Part IV: Gene Delivery Methods

11. Gene Transfer in Isolated Adult Cardiomyocytes

Kjetil Hodne, David B. Lipsett, and William E. Louch

12. Gene Transfer in Cardiomyocytes Derived from ES and iPS Cells

Francesca Stillitano, Ioannis Karakikes, and Roger J. Hajjar

13. Gene Transfer to Rodent Hearts In Vivo

Federica del Monte, Kiyotake Ishikawa, and Roger J. Hajjar

14. Ultrasound-Targeted Microbubble Destruction for Cardiac Gene Delivery

Shuyuan Chen and Paul A. Grayburn

15. A Needleless Liquid Jet Injection Delivery Approach for Cardiac Gene Therapy

Anthony S. Fargnoli, Michael G. Katz, and Charles R. Bridges

16. Cardiac Gene Delivery in Large Animal Models: Antegrade Techniques

Shin Watanabe, Lauren Leonardson, Roger J. Hajjar, and Kiyotake Ishikawa

17. Direct Myocardial Injection of Vectors

Les informations fournies dans la section � Synopsis � peuvent faire r�f�rence � une autre �dition de ce titre.

�diteur: Humana
Date d'�dition: 2018
Langue: anglais
ISBN 10: 1493982435
ISBN 13: 9781493982431
Reliure: Broch�
Nombre de pages: 368
�diteur: Ishikawa Kiyotake
Coordonn�es du fabricant: Springer Nature Customer Service Center GmbH
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Etat : New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Includes cutting-edge methods and protocols for the study of cardiac gene therapyProvides step-by-step detail essential for reproducible resultsContains key notes and implementation advice from the experts. N� de r�f. du vendeur 447957802

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Cardiac Gene Therapy: Methods and Protocols

Kiyotake Ishikawa

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Cardiac Gene Therapy | Methods and Protocols

Kiyotake Ishikawa

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Taschenbuch. Etat : Neu. Neuware -This detailed book provides methodological information on cardiac gene delivery, from classic to state-of-the-art technologies and techniques. Efficient, cardiac-specific, and safe vectors, as well as refined vector delivery methods, are key for successful cardiac gene transfer and eventually for improving patients� outcomes. Newer vectors and more efficient vector delivery methods have the potential to dramatically improve gene transduction efficacy, while novel gene manipulation techniques enforce the therapeutic power and broaden disease targets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 368 pp. Englisch. N� de r�f. du vendeur 9781493982431

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Cardiac Gene Therapy : Methods and Protocols

Kiyotake Ishikawa

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Taschenbuch. Etat : Neu. Druck auf Anfrage Neuware - Printed after ordering - This detailed book provides methodological information on cardiac gene delivery, from classic to state-of-the-art technologies and techniques. Efficient, cardiac-specific, and safe vectors, as well as refined vector delivery methods, are key for successful cardiac gene transfer and eventually for improving patients' outcomes. Newer vectors and more efficient vector delivery methods have the potential to dramatically improve gene transduction efficacy, while novel gene manipulation techniques enforce the therapeutic power and broaden disease targets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Cardiac Gene Therapy: Methods and Protocols serves as a valuable tool for molecular biologists and physiologists in the cardiology field conducting cardiac gene transfer research, which will ultimately lead to further advancements in the vital field. N� de r�f. du vendeur 9781493982431

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