Synapse Development: Methods and Protocols - Couverture souple

Couverture souple

ISBN 10 : 1493982737 ISBN 13 : 9781493982738

Editeur : Humana, 2018

Synopsis

Part I: Forming Synapses in a Dish

1. A Cell Culture System to Investigate the Presynaptic Control of Subsynaptic Membrane Differentiation at the Neuromuscular Junction

Nadine Schmidt, Sreya Basu, Stephan Köger, and Hans Rudolf Brenner

2. Co-Culture Synaptogenic Assay: A New Look at Fluorescence Reporters and Technological Devices

Karen Perez de Arce and Massimiliano Stagi

3. Synaptogenic Assays Using Neurons Cultured on Micropatterned Substrates

Katalin Czöndör and Olivier Thoumine

4. Monitoring Synapses Via Trans-Synaptic GFP Complementation

Theodoros Tsetsenis

5. Generation of Spinal Motor Neurons from Human Pluripotent Stem Cells

David P. Santos and Evangelos Kiskinis

Part II: Analyzing Synaptic Constituents

6. Biochemical Purification of Binding Partners of Synaptic Scaffold Proteins

Bekir Altas, Olaf Jahn, and Hiroshi Kawabe

7. In Situ Protein Binding Assay Using Fc-Fusion Proteins

Nirmala Padmanabhan and Tabrez J. Siddiqui

8. Reconstitution of Synaptic SNAREs into Large Liposomes with Reduced Curvature Stress

Javier M. Hernandez

9. Isolation of Synaptosomes, Synaptic Plasma Membranes, and Synaptic Junctional Complexes

Mary L. Michaelis, Lei Jiang, and Elias K. Michaelis

10. Purification of Synaptosome Populations Using Fluorescence-Activated Synaptosome Sorting

Elisa Luquet, Christoph Biesemann, Annie Munier, and Etienne Herzog

Part III: Observing Synaptic Structure and Ultrastructure

11. Optimized Protocol for Imaging Cleared Neural Tissues Using Light Microscopy

Yoh Isogai, Douglas Richardson, Catherine Dulac, and Joseph Bergan

12. Structured Illumination Microscopy for the Investigation of Synaptic Structure and Function

Soyon Hong, Daniel K. Wilton, Beth Stevens, and Douglas S. Richardson

13. 3D dSTORM Imaging of Fixed Brain Tissue

Frank Herrmannsdörfer, Benjamin Flottmann, Siddarth Nanguneri, Varun Venkataramani, Heinz Horstmann, Thomas Kuner, and Mike Heilemann

14. Photomarking Relocalization Technique for Correlated Two-Photon and Electron Microcopy Imaging of Single Stimulated Synapses

Miquel Bosch, Jorge Castro, Mriganka Sur, and Yasunori Hayashi

15. 3D Analysis of Synaptic Ultrastructure in Organotypic Hippocampal Slice Culture by High-Pressure Freezing and Electron Tomography

Cordelia Imig and Benjamin H. Cooper

Détails de l'édition

• Éditeur: Humana
• Date d'édition: 2018
• Langue: anglais
• ISBN 10: 1493982737
• ISBN 13: 9781493982738
• Reliure: Broché
• Nombre de pages: 384
• Éditeur: Poulopoulos Alexandros
• Coordonnées du fabricant: Springer Nature Customer Service Center GmbH; ProductSafety@springernature.com; ; Europaplatz 3; Heidelberg; 69115; Allemagne