Ebolaviruses: Methods and Protocols - Couverture souple

9781493983896: Ebolaviruses: Methods and Protocols

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ISBN 10 : 149398389X ISBN 13 : 9781493983896

Editeur : Humana, 2018

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Part I: Introduction

1. Marburg and Ebola Viruses: A Look Back and Lessons for the Future

Hans Dieter Klenk and Werner Slenczka

2. 40 Years of Ebolavirus Molecular Biology: Understanding a Novel Disease Agent through the Development and Application of New Technologies

Allison Groseth and Thomas Hoenen

3. Ebolavirus: An Overview of Molecular and Clinical Pathogenesis

Veronica Vine, Dana P. Scott, and Heinz Feldmann

Part II: Studying Ebolaviruses Under Biosafety Level 2 Conditions

4. Production of Filovirus Glycoprotein-Pseudotyped Vesicular Stomatitis Virus for Study of Filovirus Entry Mechanisms

Rachel B. Brouillette and Wendy Maury

5. Lentiviral Vectors Pseudotyped with Filoviral Glycoproteins

Patrick L. Sinn, Jeremy E. Coffin, Natarajan Ayithan, Kathleen H. Holt, and Wendy Maury

6. Modeling Ebola Virus Genome Replication and Transcription with Minigenome Systems

Tessa Cressey, Kristina Brauburger, and Elke M�hlberger

7. Quantification of RNA Content in Reconstituted Ebola Virus Nucleocapsids by Immunoprecipitation

Logan Banadyga and Hideki Ebihara

8. Modeling Ebolavirus Budding with Virus-Like Particles

Olivier Reynard and Mathieu Mateo

9. Modeling the Ebolavirus Life Cycle with Transcription and Replication-Competent Virus-Like Particle Assays

Nadine Biedenkopf and Thomas Hoenen

10. Assays to Measure Suppression of Type I Interferon Responses by Filovirus VP35 Proteins

Priya Luthra and Christopher Basler

11. Non-Radioactive Northern Blot Analysis to Detect Ebola Virus Minigenomic mRNA

Kristina Brauburger, Tessa Cressey, and Elke M�hlberger

Part III: Studying Infectious Ebolaviruses In Vitro

12. A Semi-Automated High Throughput Microtitration Assay for Filoviruses

Claire Marie Filone, David Miller, and Victoria Wahl-Jensen

13. Generation of Recombinant Ebola Viruses Using Reverse Genetics

Allison Groseth

14. Luciferase-Expressing Ebolaviruses as Tools for Screening of Antivirals

Thomas Hoenen

15. Live-Cell Imaging of Filoviruses

Gordian Schudt, Olga Dolnik, and Stephan Becker

16. Assessment of Inhibition of Ebola Virus Progeny Production by Antiviral Compounds

Darryl Falzarano

17. Analysis of the Cellular Stress Response During Ebola Virus Infection by Immun

Les informations fournies dans la section � Synopsis � peuvent faire r�f�rence � une autre �dition de ce titre.

�diteur: Humana
Date d'�dition: 2018
Langue: anglais
ISBN 10: 149398389X
ISBN 13: 9781493983896
Reliure: Broch�
Nombre de pages: 416
�diteur: Hoenen Thomas, Groseth Allison
Coordonn�es du fabricant: ProductSafety@springernature.com
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Ebolaviruses: Methods and Protocols

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Etat : New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Includes cutting-edge techniques for the study of ebolavirusesProvides step-by-step detail essential for reproducible resultsContains key implementation advice from the expertsIncludes cutting-edge techniques for the study of eb. N� de r�f. du vendeur 257160659

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Ebolaviruses : Methods And Protocols

Hoenen, Thomas

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Ebolaviruses | Methods and Protocols

Thomas Hoenen (u. a.)

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Ebolaviruses

Thomas Hoenen

Edit� par Humana Aug 2018, 2018

ISBN 10 : 149398389X ISBN 13 : 9781493983896

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Taschenbuch. Etat : Neu. This item is printed on demand - Print on Demand Titel. Neuware -This vital book collects key methods that have supported advancements in the field of ebolavirus molecular biology given the pressing need for the advancement of techniques for diagnostics, the development of vaccines and antivirals, and for furthering our understanding of ebolavirus biology. After an introduction, the volume delves into protocols for studying ebolavirus molecular biology under biosafety level 2 conditions, studying infectious ebolaviruses under biosafety level 4 conditions in vitro as well as in vivo, and working with ebolaviruses in the field. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Libri GmbH, Europaallee 1, 36244 Bad Hersfeld 416 pp. Englisch. N� de r�f. du vendeur 9781493983896

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Ebolaviruses : Methods and Protocols

Allison Groseth

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ISBN 10 : 149398389X ISBN 13 : 9781493983896

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Taschenbuch. Etat : Neu. Druck auf Anfrage Neuware - Printed after ordering - This vital book collects key methods that have supported advancements in the field of ebolavirus molecular biology given the pressing need for the advancement of techniques for diagnostics, the development of vaccines and antivirals, and for furthering our understanding of ebolavirus biology. After an introduction, the volume delves into protocols for studying ebolavirus molecular biology under biosafety level 2 conditions, studying infectious ebolaviruses under biosafety level 4 conditions in vitro as well as in vivo, and working with ebolaviruses in the field. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Ebolaviruses: Methods and Protocols serves as a guide to the next generations of filovirus researchers and also helps to bring experts from other areas into the filovirus research arena. N� de r�f. du vendeur 9781493983896

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