Part I: Introduction
1. Marburg and Ebola Viruses: A Look Back and Lessons for the Future
Hans Dieter Klenk and Werner Slenczka
2. 40 Years of Ebolavirus Molecular Biology: Understanding a Novel Disease Agent through the Development and Application of New Technologies
Allison Groseth and Thomas Hoenen
3. Ebolavirus: An Overview of Molecular and Clinical Pathogenesis
Veronica Vine, Dana P. Scott, and Heinz Feldmann
Part II: Studying Ebolaviruses Under Biosafety Level 2 Conditions
4. Production of Filovirus Glycoprotein-Pseudotyped Vesicular Stomatitis Virus for Study of Filovirus Entry Mechanisms
Rachel B. Brouillette and Wendy Maury
5. Lentiviral Vectors Pseudotyped with Filoviral Glycoproteins
Patrick L. Sinn, Jeremy E. Coffin, Natarajan Ayithan, Kathleen H. Holt, and Wendy Maury
6. Modeling Ebola Virus Genome Replication and Transcription with Minigenome Systems
Tessa Cressey, Kristina Brauburger, and Elke Mühlberger
7. Quantification of RNA Content in Reconstituted Ebola Virus Nucleocapsids by Immunoprecipitation
Logan Banadyga and Hideki Ebihara
8. Modeling Ebolavirus Budding with Virus-Like Particles
Olivier Reynard and Mathieu Mateo
9. Modeling the Ebolavirus Life Cycle with Transcription and Replication-Competent Virus-Like Particle Assays
Nadine Biedenkopf and Thomas Hoenen
10. Assays to Measure Suppression of Type I Interferon Responses by Filovirus VP35 Proteins
Priya Luthra and Christopher Basler
11. Non-Radioactive Northern Blot Analysis to Detect Ebola Virus Minigenomic mRNA
Kristina Brauburger, Tessa Cressey, and Elke Mühlberger
Part III: Studying Infectious Ebolaviruses In Vitro
12. A Semi-Automated High Throughput Microtitration Assay for Filoviruses
Claire Marie Filone, David Miller, and Victoria Wahl-Jensen
13. Generation of Recombinant Ebola Viruses Using Reverse Genetics
Allison Groseth
14. Luciferase-Expressing Ebolaviruses as Tools for Screening of Antivirals
Thomas Hoenen
15. Live-Cell Imaging of Filoviruses
Gordian Schudt, Olga Dolnik, and Stephan Becker
16. Assessment of Inhibition of Ebola Virus Progeny Production by Antiviral Compounds
Darryl Falzarano
17. Analysis of the Cellular Stress Response During Ebola Virus Infection by Immun
Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.
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Etat : New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Includes cutting-edge techniques for the study of ebolavirusesProvides step-by-step detail essential for reproducible resultsContains key implementation advice from the expertsIncludes cutting-edge techniques for the study of eb. N° de réf. du vendeur 257160659
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Taschenbuch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This vital book collects key methods that have supported advancements in the field of ebolavirus molecular biology given the pressing need for the advancement of techniques for diagnostics, the development of vaccines and antivirals, and for furthering our understanding of ebolavirus biology. After an introduction, the volume delves into protocols for studying ebolavirus molecular biology under biosafety level 2 conditions, studying infectious ebolaviruses under biosafety level 4 conditions in vitro as well as in vivo, and working with ebolaviruses in the field. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Ebolaviruses: Methods and Protocols serves as a guide to the next generations of filovirus researchers and also helps to bring experts from other areas into the filovirus research arena. 416 pp. Englisch. N° de réf. du vendeur 9781493983896
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Taschenbuch. Etat : Neu. Druck auf Anfrage Neuware - Printed after ordering - This vital book collects key methods that have supported advancements in the field of ebolavirus molecular biology given the pressing need for the advancement of techniques for diagnostics, the development of vaccines and antivirals, and for furthering our understanding of ebolavirus biology. After an introduction, the volume delves into protocols for studying ebolavirus molecular biology under biosafety level 2 conditions, studying infectious ebolaviruses under biosafety level 4 conditions in vitro as well as in vivo, and working with ebolaviruses in the field. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Ebolaviruses: Methods and Protocols serves as a guide to the next generations of filovirus researchers and also helps to bring experts from other areas into the filovirus research arena. N° de réf. du vendeur 9781493983896
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Taschenbuch. Etat : Neu. Neuware -This vital book collects key methods that have supported advancements in the field of ebolavirus molecular biology given the pressing need for the advancement of techniques for diagnostics, the development of vaccines and antivirals, and for furthering our understanding of ebolavirus biology. After an introduction, the volume delves into protocols for studying ebolavirus molecular biology under biosafety level 2 conditions, studying infectious ebolaviruses under biosafety level 4 conditions in vitro as well as in vivo, and working with ebolaviruses in the field. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 416 pp. Englisch. N° de réf. du vendeur 9781493983896
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