Part I: Review and Overview Chapters
1. Master Regulatory Transcription Factors in Plant Development: A Blooming Perspective
Kerstin Kaufmann and Chiara A. Airoldi
2. Application of CRISPR/Cas to Understand Cis- and Trans-Regulatory Elements in Plants
Felix Wolter and Holger Puchta
3. The Long-Term 'In Natura' Study Sites of Arabidopsis halleri for Plant Transcription and Epigenetic Modification Analyses in Natural Environments
Hiroshi Kudoh, Mie N. Honjo, Haruki Nishio, and Jiro Sugisaka
Part II: Preparation of Plant Materials
4. Generation of Inducible Transgenic Lines of Arabidopsis Transcription Factors Regulated by MicroRNAs
Pooja Aggarwal, Krishna Reddy Challa, Monalisha Rath, Preethi Sunkara, and Utpal Nath
5. A Specific Knockdown of Transcription Factor Activities in Arabidopsis
Beibei Zheng, Bennett Thomson, and Frank Wellmer
6. Using CRISPR/Cas9 System to Introduce Targeted Mutation in Arabidopsis
Ze Hong Lee, Nobutoshi Yamaguchi, and Toshiro Ito
7. CRISPR/Cas9-Based Genome Editing of Transcription Factor Genes in Marchantia polymorpha
Shigeo S. Sugano and Ryuichi Nishihama
8. Cell-Type-Specific Promoter Identification Using Enhancer Trap Lines
Tatyana Radoeva, Shunsuke Saiga, and Dolf Weijers
9. Isolation of Arabidopsis Palisade and Spongy Mesophyll Cells
Kyohei Uemoto, Takashi Araki, and Motomu Endo
10. Ectopic Vascular Induction in Arabidopsis Cotyledons for Sequential Analysis of Phloem Differentiation
Alif Meem Nurani, Yuki Kondo, and Hiroo Fukuda
Part III: Detection of Expression Levels
11. High Impact Gene Discovery: Simple Strand Specific mRNA Library Construction and Differential Regulatory Analysis Based on Gene Co-Expression Network
Yasunori Ichihashi, Atsushi Fukushima, Arisa Shibata, and Ken Shirasu
12. Laser Capture Micro-Dissection Coupled to RNA Sequencing: A Powerful Approach Applied to the Model Legume Medicago truncatula in Interaction with Sinorhizobium meliloti
Brice Roux, Nathalie Rodde, Sandra Moreau, Marie-Françoise Jardinaud, and Pascal Gamas
13. nanoCAGE-XL: An Approach to High-Confidence Transcription Start Site Sequencing
Maria G. Ivanchenko and Molly Megraw
14. Genome-Wide TSS Identification in Maize
María Katherine Mejia-Guerra, Wei Li, Andrea I. Doseff, and Erich Grotewold
15. Three-Dimensional Multiphoton Imaging of Transcription Factor by ClearSee
&nb
Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.
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Etat : New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Includes cutting-edge methods and protocols for the study of plant transcription factorsProvides step-by-step detail essential for reproducible resultsContains key notes and implementation advice from the expert. N° de réf. du vendeur 220546638
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Buch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed book provides general protocols and technologies that plant biologists worldwide often utilize for the purpose of accelerating research progress in the field of plant transcription factors. Beginning with a brief introduction, the volume continues by exploring methods in the preparation of plant materials, detection of expression levels, interaction tests, and chromatin analyses. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Plant Transcription Factors: Methods and Protocols aims to answer a wide range of questions related to transcription factors commonly raised by plant biologists. 408 pp. Englisch. N° de réf. du vendeur 9781493986569
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Buch. Etat : Neu. This item is printed on demand - Print on Demand Titel. Neuware -This detailed book provides general protocols and technologies that plant biologists worldwide often utilize for the purpose of accelerating research progress in the field of plant transcription factors. Beginning with a brief introduction, the volume continues by exploring methods in the preparation of plant materials, detection of expression levels, interaction tests, and chromatin analyses. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 408 pp. Englisch. N° de réf. du vendeur 9781493986569
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Buch. Etat : Neu. Druck auf Anfrage Neuware - Printed after ordering - This detailed book provides general protocols and technologies that plant biologists worldwide often utilize for the purpose of accelerating research progress in the field of plant transcription factors. Beginning with a brief introduction, the volume continues by exploring methods in the preparation of plant materials, detection of expression levels, interaction tests, and chromatin analyses. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Plant Transcription Factors: Methods and Protocols aims to answer a wide range of questions related to transcription factors commonly raised by plant biologists. N° de réf. du vendeur 9781493986569
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