1. Microinjection and Micromanipulation: A Historical Perspective
Wenhao Xu
2. Production of Transgenic Mice by Pronuclear Microinjection
Xin-an Pu, Anthony P. Young, and H. Michael Kubisch
3. Transgene Recombineering in Bacterial Artificial Chromosomes
Michael G. Zeidler and Thomas L. Saunders
4. Using TARGATTTM Technology to Generate Site-Specific Transgenic Mice
Ruby Yanru Chen-Tsai
5. Generating Genetically Engineered Mice Using a Spermatogonial Stem Cell-Mediated Method
Takuya Sato and Takehiko Ogawa
6. Chimeric Mouse Generation by ES Cell Blastocyst Microinjection and Uterine Transfer
Yubin Du, Wen Xie, Fan Zhang, and Chengyu Liu
7. Creating Knock-In Alleles in Mouse Embryonic Stem Cells by CRISPR/Cas9-Mediated Homologous Recombination without Drug Selection
Pinghu Liu, Yan Li, Jingqi Lei, and Lijin Dong
8. Using CRISPR/Cas9 for Gene Knockout in Immunodeficient NSG Mice
Yubin Du, Wen Xie, Fan Zhang, Uimook Choi, Chengyu Liu, and Colin L. Sweeney
9. Generation of CRISPR-Edited Rodents Using a Piezo-Driven Zygote Injection Technique
Melissa A. Scott and Yueh-Chiang Hu
10. Delivery of CRISPR-Cas9 into Mouse Zygotes by Electroporation
Wenning Qin and Haoyi Wang
11. Generation of Conditional Knockout Mice by Sequential Insertion of Two loxP Sites In Cis Using CRISPR/Cas9 and Single-Stranded DNA Oligonucleotides
Ye Liu, Yubin Du, Wen Xie, Fan Zhang, Douglas Forrest, and Chengyu Liu
12. Improvement of Mouse Cloning from Any Type of Cell by Nuclear Injection
Sayaka Wakayama, Satoshi Kishigami, and Teruhiko Wakayama
13. The CARD Method for Simple Vitrification of Mouse Oocytes: Advantages and Applications
Naomi Nakagata, Jorge Sztein, and Toru Takeo
14. The CARD Method for Mouse Sperm Cryopreservation and In Vitro Fertilization Using Frozen-Thawed Sperm
Toru Takeo, Jorge Sztein, and Naomi Nakagata
15. Isolation and Analysis of a Genome-Edited Single-Hepatocyte from a Cas9 Transgenic Mouse Line
Takayuki Sakurai, Akiko Kamiyoshi, Masato Ohtsuka, Channabasavaiah B. Gurumurthy, Masahiro Sato, and Takayuki Shindo
16. Microinjection and Oviduct Transfer Procedures for Rat Model Generation with CRISPR-Cas9 Technology
Tuija M. Alcantar and Xin Y. Rairdan
17. Molecular Aspects of Zinc Finger Nucleases (ZFNs)-Mediated Gene Editing in Rat Embryos
&nbs
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Buch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed book explores how microinjection will be used in the foreseeable future, not only for generating animal models for biomedical research but also for changing economically or ecologically important species that can broadly impact our society in general. The opening half of the book focuses on methods for generating mouse models, as they are still the most popular in genome engineering research, while the second half examines gene-editing in a variety of other species, opened up by the developments in ZFN, TALEN, and CRISPR techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Microinjection: Methods and Protocols serves as an ideal guide for researchers looking to take advantage of the breakthrough technologies in gene-editing and embryo micromanipulations. 556 pp. Englisch. N° de réf. du vendeur 9781493988303
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Buch. Etat : Neu. This item is printed on demand - Print on Demand Titel. Neuware -This detailed book explores how microinjection will be used in the foreseeable future, not only for generating animal models for biomedical research but also for changing economically or ecologically important species that can broadly impact our society in general. The opening half of the book focuses on methods for generating mouse models, as they are still the most popular in genome engineering research, while the second half examines gene-editing in a variety of other species, opened up by the developments in ZFN, TALEN, and CRISPR techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 556 pp. Englisch. N° de réf. du vendeur 9781493988303
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