Part I. Metalloproteins
1. Nitrogenases
Nate Sickerman, Yilin Hu, and Markus W. Ribbe
2. Bacteriochlorophyll and Coenzyme F430
Jürgen Moser and Gunhild Layer
3. Carbon Monoxide Dehydrogenases
Jae-Hun Jeoung, Berta M. Martins, and Holger Dobbek
4. Molybdenum-Containing Enzymes
Dimitri Niks and Russ Hille
5. Hydrogenases
Nathaniel S. Sickerman and Yilin Hu
Part II. Genetic and Biochemical Methods
6. Genomic Manipulations of the Diazotroph Azotobacter vinelandii
Patricia Dos-Santos
7. Purification of Nitrogenase Proteins
Chi Chung Lee, Markus W. Ribbe, and Yilin Hu
8. Expression, Purification and Activity Analysis of Chlorophyllide Oxidoreductase and Ni2+-Sirohydrochlorin a, c-Diamide Reductase
Jürgen Moser, Jan Jasper, José Vazquez Ramos, Sven T. Sowa, and Gunhild Layer
9. Reconstitution of Molybdoenzymes with Bis-Molybdopterin Guanine Dinucleotide Cofactor
Paul Kaufmann, Chantal Iobbi-Nivol, and Silke Leimkühler
Part III. Structural and Spectroscopic Methods
10. Crystallization of Nitrogenase Proteins
Belinda B. Wenke, Renee J. Arias, and Thomas Spatzal
11. X-Ray Crystallography of Carbon Monoxide Dehydrogenases
Jae-Hun Jeoung, Berta M. Martins, and Holger Dobbek
12. X-Ray Absorption Spectroscopy of Metalloproteins
Limei Zhang
13. Electron Paramagnetic Resonance Spectroscopy of Metalloproteins
Andrew Jasniewski, Yilin Hu, and Markus Ribbe
14. Magnetic Circular Dichroism Spectroscopy of Metalloproteins
Brian J. Hales
Part IV. Chemical methods and Theoretical calculations
15. Chemical Synthesis of an Asymmetric Mimic of the Nitrogenase Active Site
Kazuki Tanifuji and Yasuhiro Ohki
16. Computational Methods for Modeling Metalloproteins
Martin Stiebritz and Yilin Hu
Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.
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Gebunden. Etat : New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the expertsIncludes cutting-ed. N° de réf. du vendeur 235194318
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Buch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume provides an up-to-date, in-depth overview of the methods that have been applied to studying the complex metalloproteins at a molecular level. Chapters cover a wide range of approaches focusing on genetic, biochemical, spectroscopic, chemical methods, and theoretical calculations. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Metalloproteins: Methods and Protocols aims to be useful for anyone who is interested in metalloprotein research and wants to address the unanswered mechanistic and biosynthetic questions of these fascinating enzyme systems. 288 pp. Englisch. N° de réf. du vendeur 9781493988631
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Buch. Etat : Neu. Druck auf Anfrage Neuware - Printed after ordering - This volume provides an up-to-date, in-depth overview of the methods that have been applied to studying the complex metalloproteins at a molecular level. Chapters cover a wide range of approaches focusing on genetic, biochemical, spectroscopic, chemical methods, and theoretical calculations. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Metalloproteins: Methods and Protocols aims to be useful for anyone who is interested in metalloprotein research and wants to address the unanswered mechanistic and biosynthetic questions of these fascinating enzyme systems. N° de réf. du vendeur 9781493988631
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