The Hippo Pathway: Methods and Protocols - Couverture rigide

Couverture rigide

ISBN 10 : 149398909X ISBN 13 : 9781493989096

Editeur : Humana Press Inc., 2018

Synopsis

Part I: The Hippo Pathway: Drosophila Genetics Lead the Way

1. The Power of Drosophila Genetics: The Discovery of the Hippo Pathway

Rewatee Gokhale and Cathie M. Pfleger

2. Drosophila Genetics: The Power of Genetic Mosaic Approaches

Mardelle Atkins

3. Drosophila Genetics: Analysis of Tissue Growth in Adult Tissues

Alexander D. Fulford and Paulo S. Ribeiro

4. Live Imaging of Hippo Pathway Components in Drosophila Imaginal Discs

Jiajie Xu, Ting Su, Sherzod A. Tokamov, and Richard G. Fehon

5. Localization of Hippo Signaling Components in Drosophila by Fluorescence and Immunofluorescence

Cordelia Rauskolb and Kenneth D. Irvine

6. Bimolecular Fluorescence Complementation (BiFC) in Tissue Culture and in Developing Tissues of Drosophila to Study Protein-Protein Interactions

Yurika Matsui and Zhi-Chun Lai

Part II: Molecular and Cell Biology Studies of the Hippo Pathway

7. Immunohistochemistry to Study YAP in Human Tissue Samples

Franziska Haderk, Victor Olivas, and Trever G. Bivona

8. Immunofluorescence Study of Endogenous YAP in Mammalian Cells

Valentina Rausch and Carsten G. Hansen

9. Immunofluorescence Microscopy to Study Endogenous TAZ in Mammalian Cells

Nathan M. Kingston, Andrew M. Tilston-Lunel, Julia Hicks-Berthet, and Xaralabos Varelas

10. Nuclear/Cytoplasmic Fractionation to Study Hippo Effectors

Maria Chatzifrangkeskou and Eric O'Neill

11. Luciferase Reporter Assays to Determine YAP/TAZ Activity in Mammalian Cells

Sirio Dupont

12. Quantitative Real-Time PCR to Measure YAP/TAZ Activity in Human Cells

Xiaolei Cao and Bin Zhao

13. HTRF(R) Total and Phospho-YAP(Ser127) Cellular Assays

Diana Zindel, Claire Vol, Odile Lecha, Isabelle Bequignon, Merve Bilgic, Marion Vereecke, Fabienne Charrier-Savournin, Maïté Romier, Eric Trinquet, Jean-Philippe Pin, Julie Pannequin, Thomas Roux, Elodie Dupuis, and Laurent Prézeau

14. Studying YAP-Mediated 3D Morphogenesis Using Fish Embryos and Human Spheroids

Yoichi Asaoka, Hitoshi Morita, Hiroko Furumoto, Carl-Philipp Heisenberg, and Makoto Furutani-Seiki

15. Regulation of YAP/TAZ Activity by Mechanical Cues: An Experimental Overview

Sirio Dupont

16. CRISPR-Mediated Approaches to Regulate YAP/TAZ Levels

Ryan J. Quinton and Neil J. Ganem

17. Hippo Pathway Reg

Détails de l'édition

• Éditeur: Humana Press Inc.
• Date d'édition: 2018
• Langue: anglais
• ISBN 10: 149398909X
• ISBN 13: 9781493989096
• Reliure: Relié
• Nombre de pages: 388
• Éditeur: Hergovich Alexander
• Coordonnées du fabricant: Springer Nature Customer Service Center GmbH; ProductSafety@springernature.com; ; Europaplatz 3; Heidelberg; 69115; Allemagne