Part I: Astrocyte Biology: Overviews and Perspectives
1. The Astrocyte-Neuron Interface: An Overview on Molecular and Cellular Dynamics Controlling Formation and Maintenance of the Tripartite Synapse
Uzma Hasan and Sandeep K. Singh
2. Role of Astrocytes in the Neurogenic Niches
Julia Schneider, Julian Karpf, and Ruth Beckervordersandforth
Part II: Isolation of Astrocytes from Animal Models
3. Isolation and Culture of Astrocytes from Postnatal and Adult Mouse Brains
Yingbo He, Natalie Taylor, and Anindya Bhattacharya
4. Fluorescence-Activated Cell Sorting-Based Isolation and Characterization of Neural Stem Cells from the Adult Zebrafish Telencephalon
Rossella Di Giaimo, Sven Aschenbroich, and Jovica Ninkovic
Part III: Astrocyte Morphology and Functions: Emerging Methods for Their Characterizations In Vivo, In Situ, and In Vitro
5. A Method to Visualize the Nanoscopic Morphology of Astrocytes In Vitro and In Situ
Janosch P. Heller and Dmitri A. Rusakov
6. A Specialized Method to Resolve Fine 3D Features of Astrocytes in Non-Human Primate (Marmoset, Callithrix Jacchus) and Human Fixed Brain Samples
Gaël Quesseveur, Aymeric Fouquier d'Hérouël, Keith K. Murai, and David S. Bouvier
7. Cisterna Magna Injection in Rats to Study Glymphatic Function
Marta Ramos, Nicholas Burdon Bechet, Roberta Battistella, Chiara Pavan, Anna L.R. Xavier, Maiken Nedergaard, and Iben Lundgaard
8. Preparing the Astrocyte Perivascular Endfeet Transcriptome to Investigate Astrocyte Molecular Regulations at the Brain Vascular Interface
Anne-Cécile Boulay, Noémie Mazaré, Bruno Saubaméa, and Martine Cohen-Salmon
9. Monitoring Interneuron-Astrocyte Signaling and Its Consequences on Synaptic Transmission
Sara Mederos and Gertrudis Perea
10. Astrocytes and Circadian Rhythms: An Emerging Astrocyte-Neuron Synergy in the Timekeeping System
Olga Barca Mayo, Luca Berdondini, and Davide De Pietri Tonelli
11. In Vitro Engulfment Assay to Measure Phagocytic Activity of Astrocytes Using Synaptosomes
Youkyeong Gloria Byun and Won-Suk Chung
12. Quantifying Compartment-Specific Protein Translocation in Astrocytes by Object-Oriented Image Analysis: Mitochondrial Translocation of PKCδ
Kiavasch M.N. Farid and Amin Derouiche
13. Imaging of Local and Global Sodium Signals in Astrocytes
Niklas J. Gerkau, Karl W. Kafitz, and Christine R. Rose
14. Detection of Protein Uptake in In Vitro Cultured Astrocytes Exemplified by the Uptake of the Serine Protease, Tissue Plasminogen Activator
Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.
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Etat : New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Includes cutting-edge techniquesProvides step-by-step detail essential for reproducible resultsContains key implementation advice from the expertsThis detailed volume gathers together a broad varie. N° de réf. du vendeur 251556727
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Buch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed volume gathers together a broad variety of methods essential to the investigation of the biology of astrocytes and their multifaceted roles in both healthy and diseased brains. Beginning with some overviews of the subject, the book continues by covering techniques for the isolation of astrocytes from animal models, the investigation of astrocyte morphology and function, as well as for understanding astrocyte pathologies in the central nervous system. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Astrocytes: Methods and Protocols serves as an ideal guide for both experienced and beginner scientists working toward unraveling the novel, fascinating roles of these versatile cells. 308 pp. Englisch. N° de réf. du vendeur 9781493990672
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Buch. Etat : Neu. Astrocytes | Methods and Protocols | Barbara Di Benedetto | Buch | xi | Englisch | 2019 | Humana | EAN 9781493990672 | Verantwortliche Person für die EU: Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin, juergen[dot]hartmann[at]springer[dot]com | Anbieter: preigu Print on Demand. N° de réf. du vendeur 114825983
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