Articles liés à CRISPR Gene Editing: Methods and Protocols

CRISPR Gene Editing: Methods and Protocols - Couverture rigide

 
9781493991693: CRISPR Gene Editing: Methods and Protocols
Couverture rigide
ISBN 10 : 1493991698 ISBN 13 : 9781493991693
Editeur : Humana Press Inc., 2019

Synopsis

This detailed volume guides readers through strategic planning and user-friendly guidelines in order to select the most suitable CRISPR-Cas system and target sites with high activity and specificity. Methods covering CRISPR gRNA design, CRISPR delivery, CRISPR activity quantification (indel quantification), and examples of applying CRISPR gene editing in human pluripotent stem cells, primary cells, gene therapy, and genetic screening are included. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and invaluable, CRISPR Gene Editing: Methods and Protocols will assist undergraduates, graduates, and researchers with detailed guidelines and methods for the vitally important CRISPR gene editing field.
Chapter 3 is available open access under a CC BY 4.0 license via link.springer.com.

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Éditeur
Humana Press Inc.
Date d'édition
2019
Langue
anglais
ISBN 10 
1493991698
ISBN 13 
9781493991693
Reliure
Relié
Numéro d'édition
1
Nombre de pages
373
Éditeur
Luo Yonglun
Coordonnées du fabricant
GPSR Kontakt
ProductSafety@springernature.com

Abraham-Lincoln-Str. 46
Wiesbaden
65189
Allemagne