A comprehensive collection of cutting-edge methods to study and work with the vaccinia virus and other poxvirus gene transcriptions. These readily reproducible techniques can be used for the construction and characterization of recombinant viruses; for the study of poxvirus gene transcription and DNA replication; for the investigation of the binding, entry, and movement of the virus in host cells; and for the use of the virus in immunologic and cell-cell fusion assays. Other methods cover poxvirus bioinformatics, aspects of viral pathogenesis at both a protein and an animal model level, and the study of immune responses to poxviruses - the latter a critical ability given the important role of vaccinia virus in smallpox vaccination and their potential role as vaccine vectors directed against infectious agents and cancer.
Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.
The second edition of this Methods in Molecular Biology volume details important progress in poxvirology, and offers entirely new sets of protocols. Includes materials and reagents lists, step-by-step lab protocols, and tips on pitfalls and troubleshooting.
Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.
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