Oligonucleotide Synthesis: Methods and Applications (288) - Couverture rigide

Couverture rigide

ISBN 10 : 1588292339 ISBN 13 : 9781588292339

Editeur : Humana Press Inc., 2004

Synopsis

A collection of powerful new techniques for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are a novel two-step process that yields a high purity, less costly, DNA, the synthesis of phosphorothioates using new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, and triple helix formation. The applications include using molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules of DNA, nucleic acid libraries, and the use of small interference RNA (siRNA) as an inhibitor of gene expression.

Détails de l'édition

• Éditeur: Humana Press Inc.
• Date d'édition: 2004
• Langue: anglais
• ISBN 10: 1588292339
• ISBN 13: 9781588292339
• Reliure: Relié
• Nombre de pages: 452
• Éditeur: Herdewijn Piet
• Coordonnées du fabricant: Springer Nature Customer Service Center GmbH; ProductSafety@springernature.com; ; Europaplatz 3,69115 Heidelberg, Germany; Heidelberg; 69115; Allemagne