John Walker and Ralph Rapley have collected a wide-ranging group of molecular and biochemical techniques that are the most frequently used in medical and clinical research, especially diagnostics. The authors-well-established investigators who run their own research programs and use the methods on a regular basis-outline the practical procedures for using them and describe a variety of pertinent applications. Among the technologies presented are southern and western blotting, electrophoresis, PCR, cDNA and protein microarrays, liquid chromatography, in situ hybridization, karyotyping, flow cytometry, bioinformatics, genomics, and ribotyping. The applications include assays for mutation detection, mRNA analysis, chromosome translocations, inborn errors of metabolism, protein therapeutics, and gene therapy.
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John Walker and Ralph Rapley have collected a wide-ranging group of molecular and biochemical techniques that are the most frequently used in medical and clinical research, especially diagnostics. The authors-well-established investigators who run their own research programs and use the methods on a regular basis-outline the practical procedures for using them and describe a variety of pertinent applications. Among the technologies presented are southern and western blotting, electrophoresis, PCR, cDNA and protein microarrays, liquid chromatography, in situ hybridization, karyotyping, flow cytometry, bioinformatics, genomics, and ribotyping. The applications include assays for mutation detection, mRNA analysis, chromosome translocations, inborn errors of metabolism, protein therapeutics, and gene therapy.
The numerous advances in our ability to investigate and define cellular processes at the molecular level, have been the result of the development and application of powerful new techniques in molecular biology, in particular nucleic acid and protein methodologies. The purpose of Medical Biomethods Handbook is to introduce the reader to a wide-ranging selection of those molecular biology techniques that are most frequently used by research workers in the field of medical and clinical research. The editors have detailed the theory behind, and the application of, the techniques described. This Handbook will prove invaluable to medical researchers, students and professors who wish to understand and use new techniques, but do not yet have the necessary background to set them up.
Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.
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