In Situ Hybridization Protocols - Couverture rigide

Couverture rigide

ISBN 10 : 1588294021 ISBN 13 : 9781588294029

Editeur : Humana Press Inc., 2005

Synopsis

In Situ Hybridization has successfully provided basic and advanced laboratory techniques. With increasing technology and evolving applications, this edition concentrates on in situ hybridization of cells and tissues and the derivative techniques that have not been covered in the previous editions. The third edition will be a valuable aid in setting up the technique or improving the sensitivity and scope of applications of in situ hybridization in their own laboratories.

A propos du livre

À propos de la quatrième de couverture

In In Situ Hybridization, Third Edition, Ian Darby updates his highly successful second edition and shifts the focus to tissue and cell in situ hybridization. Drawing on experts working in diverse areas and taking advantage of advances in non-isotopic in situ hybridization and amplification techniques, the authors describe in detail their readily reproducible techniques for preparation, detection, and tissue hybridization. Among the new techniques detailed are PNA probes for viral diagnostics, plant in situ hybridization, cell proliferation detection, and quantitation of in situ hybridization. There are also cutting-edge techniques for tissue microarrays, expanded embryology-developmental gene detection, and expanded cell culture. Derivative techniques presented include the identification of transplanted cells, histones, nick-end labeling for apoptosis, the use of peptide nucleic acid probes, and in situ hybridization of plant specimens. The protocols follow the successful Methods in Molecular Biology(TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Completely updated and highly practical, In Situ Hybridization, Third Edition allows both the molecular biologist with little experience of histology and the histologist with little experience of molecular biology to use these powerful new techniques successfully.

Détails de l'édition

• Éditeur: Humana Press Inc.
• Date d'édition: 2005
• Langue: anglais
• ISBN 10: 1588294021
• ISBN 13: 9781588294029
• Reliure: Relié
• Numéro d'édition: 3
• Nombre de pages: 270
• Éditeur: Darby Ian A, Hewitson Tim D
• Coordonnées du fabricant: Springer Nature Customer Service Center GmbH; ProductSafety@springernature.com; ; Europaplatz 3; Heidelberg; 69115; Allemagne