Handbook of ELISPOT: Methods and Protocols - Couverture rigide

Couverture rigide

ISBN 10 : 1588294692 ISBN 13 : 9781588294692

Editeur : Humana Press Inc., 2005

Synopsis

In this first book dedicated entirely to the ELISPOT, a critical enzyme-linked immunospot assay used widely in biomedical research, recognized experts with first-hand experience detail how to design, perform, and analyze these assays. The readily reproducible techniques they provide cover a wide variety of topics, including the use of membrane-backed plates, the standardization and validation procedures, the removal of cells from ELISPOT plates, cell separation techniques, and the quantification of ELISPOT data. There are also numerous ELISPOT applications involving animal models, human cells, measles, multiple sclerosis, immune responses, multicytokine detection systems, and immunocytochemistry. Highlights include dual-color and multiplex ELISPOT assays, use of the ELISPOT assay on feline lymphocytes, standardization of the ELISPOT procedure, and combining the ELISPOT assay with immunohistochemistry.

A propos du livre

Présentation de l'éditeur

Straight from the labs of seasoned experts, this book covers setting and performing ELISPOT assays, ELISPOT for veterinary research, advanced ELISPOT techniques, image and data analysis, as well as vaccine development and diagnostics.

Détails de l'édition

• Éditeur: Humana Press Inc.
• Date d'édition: 2005
• Langue: anglais
• ISBN 10: 1588294692
• ISBN 13: 9781588294692
• Reliure: Relié
• Nombre de pages: 336
• Éditeur: Kalyuzhny Alexander E.
• Coordonnées du fabricant: non disponible
• Personne responsable: non disponible