The second edition of Adhesion Protein Protocols combines traditional techniques with cutting-edge and new techniques that can be adapted easily to different molecules and cell types. The topics discussed in this updated second edition include novel techniques for studying cell-cell adhesion, neutrophil chemotaxis, in vitro assays used to study leukocyte migration through monolayers of cultured endothelial cells, and novel techniques to purify pseudopodia from migratory cells. This book also discusses the study of cell-matrix interaction, RNA interference, fluorescence recovery after photobleaching, actin purification, the application of microarray techniques, and the role of adhesion proteins in the study of proteomics. The protocols discussed in this volume are suitable for both novice and expert scientists, who will gain further insight into the complex and incompletely understood processes involved in cellular adhesion.
Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.
This book covers techniques in cellular adhesion. It features lists of the necessary materials and reagents; step-by-step, readily reproducible protocols; and notes on troubleshooting and avoiding known pitfalls.
Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.
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