Drug-DNA Interaction Protocols (613) - Couverture rigide

Couverture rigide

ISBN 10 : 1603274170 ISBN 13 : 9781603274173

Editeur : Humana Press Inc., 2009

Synopsis

DNA has been known to be the cellular target for many cytotoxic anticancer agents for several decades. The knowledge of its structure in atomic detail and the ease with which DNA fragments (both synthetic oligonucleotides and natural sequences) can be prepared and manipulated has aided the design of compounds that bind to it with improved sel- tivity. On the basis of this information, new generations of sequence reading compounds (including triplex forming oligonucleotides and minor groove binding ligands) have been prepared, which have the potential for targeting specifc DNA sequences as anti-gene agents. Within the last 10 years, it has also become apparent that the familiar DNA duplex is not the only structure that can be targeted by DNA-binding ligands and there has been increased interest in triplex and quadruplex structures as drug targets, as well as protein- DNA complexes, such as those with nucleosomes or topoisomerases. Each of these advances has required the availability and development of an arsenal of techniques for probing the interactions in both qualitative and quantitative terms. This v- ume of Methods in Molecular Biology brings together several techniques that are currently useful for examining these interactions. Some of these are updates on ones that were included in the earlier volume (Methods in Molecular Biology 90), published 12 years ago, while others are new.

A propos du livre

Présentation de l'éditeur

The last few years have witnessed the creation of new generations of sequence reading compounds, which have incredible potential for targeting specific DNA sequences. In this authoritative and current book, expert researchers explore these compounds.

Détails de l'édition

• Éditeur: Humana Press Inc.
• Date d'édition: 2009
• Langue: anglais
• ISBN 10: 1603274170
• ISBN 13: 9781603274173
• Reliure: Relié
• Numéro d'édition: 2
• Nombre de pages: 320
• Éditeur: Fox Keith R.
• Coordonnées du fabricant: ProductSafety@springernature.com; ProductSafety@springernature.com; ; Poststr. 9; Darmstadt; 64293; Allemagne