Surface Plasmon Resonance: Methods and Protocols - Couverture rigide

Couverture rigide

ISBN 10 : 1607616696 ISBN 13 : 9781607616696

Editeur : Humana Press Inc., 2010

Synopsis

Surface plasmon resonance (SPR) has evolved into an exciting technique in biomolecular interaction analysis. The development of commercial SPR instruments has made the te- nique available to a wide scienti?c audience, and the number of publications in which the use of SPR is described is rapidly increasing. SPR is in use for many purposes from food quality control to the study of nanoparticles. Much research is now focused on devel- ing new SPR-related applications, e.g., SPR imaging, SPR arrays, SPR ?uorescence, and combinations of SPR with mass spectrometry and with electrochemistry. Biomolecular interaction analysis is at the core of many research projects. In principle, the setup of an SPR experiment is simple: There is a sensor surface to which one of the interacting partners (the ligand) is immobilized; the other partner (the analyte) is added in a ?ow or cell-like compartment. The binding phenomenon is monitored in real time as a change in SPR angle. An important issue is the choice of surface and the immobilization strategy. With SPR, it is possible to mimic the biological environment which is relevant for an interaction. For interactions in a water environment, sensor surfaces with hydrogels are available. Many biomolecular interactions take place in a membrane environment. For this, commercial sensor surfaces are available, or surfaces can be tailor-made. This volume contains several examples of building up of lipophilic surfaces. Nature abundantly makes use of multivalent interactions; multivalency can be mimicked on a sensor surface with immobilized ligands.

Détails de l'édition

• Éditeur: Humana Press Inc.
• Date d'édition: 2010
• Langue: anglais
• ISBN 10: 1607616696
• ISBN 13: 9781607616696
• Reliure: Relié
• Numéro d'édition: 1
• Nombre de pages: 296
• Éditeur: De Mol Nico J., Fischer Marcel J. E.
• Coordonnées du fabricant: ProductSafety@springernature.com; ProductSafety@springernature.com; ; Poststr. 9; Darmstadt; 64293; Allemagne