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9781617375316: Fluorescent Energy Transfer Nucleic Acid Probes: Designs and Protocols

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ISBN 10 : 1617375314 ISBN 13 : 9781617375316

Editeur : Humana, 2010

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Fluorescent nucleic acid probes, which use energy transfer, include such constructs as molecular beacons, molecular break lights, Scorpion primers, TaqMan probes, and others. These probes signal detection of their targets by changing either the intensity or the color of their fluorescence. Not surpr- ingly, these luminous, multicolored probes carry more flashy names than their counterparts in the other fields of molecular biology. In recent years, fluor- cent probes and assays, which make use of energy transfer, have multiplied at a high rate and have found numerous applications. However, in spite of this explosive growth in the field, there are no manuals summarizing different p- tocols and fluorescent probe designs. In view of this, the main objective of Fluorescent Energy Transfer Nucleic Acid Probes: Designs and Protocols is to provide such a collection. Oligonucleotides with one or several chromophore tags can form fluor- cent probes capable of energy transfer. Energy transport within the probe can occur via the resonance energy transfer mechanism, also called F�rster tra- fer, or by non-F�rster transfer mechanisms. Although the probes using F�rster transfer were developed and used first, the later non-F�rster-based probes, such as molecular beacons, now represent an attractive and widely used option. The term "fluorescent energy transfer probes" in the title of this book covers both F�rster-based fluorescence resonance energy transfer (FRET) probes and probes using non-FRET mechanisms. Energy transfer probes serve as molecule-size sensors, changing their fluorescence upon detection of various DNA reactions.

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�diteur: Humana
Date d'�dition: 2010
Langue: anglais
ISBN 10: 1617375314
ISBN 13: 9781617375316
Reliure: Broch�
Nombre de pages: 388
�diteur: Didenko Vladimir V.
Coordonn�es du fabricant: Springer Nature Customer Service Center GmbH
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Fluorescent Energy Transfer Nucleic Acid Probes (eng)

Didenko, Vladimir V.

Edit� par Humana, 2010

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Fluorescent Energy Transfer Nucleic Acid Probes

Vladimir V. Didenko

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Taschenbuch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Fluorescent nucleic acid probes, which use energy transfer, include such constructs as molecular beacons, molecular break lights, Scorpion primers, TaqMan probes, and others. These probes signal detection of their targets by changing either the intensity or the color of their fluorescence. Not surpr- ingly, these luminous, multicolored probes carry more flashy names than their counterparts in the other fields of molecular biology. In recent years, fluor- cent probes and assays, which make use of energy transfer, have multiplied at a high rate and have found numerous applications. However, in spite of this explosive growth in the field, there are no manuals summarizing different p- tocols and fluorescent probe designs. In view of this, the main objective of Fluorescent Energy Transfer Nucleic Acid Probes: Designs and Protocols is to provide such a collection. Oligonucleotides with one or several chromophore tags can form fluor- cent probes capable of energy transfer. Energy transport within the probe can occur via the resonance energy transfer mechanism, also called F�rster tra- fer, or by non-F�rster transfer mechanisms. Although the probes using F�rster transfer were developed and used first, the later non-F�rster-based probes, such as molecular beacons, now represent an attractive and widely used option. The term 'fluorescent energy transfer probes' in the title of this book covers both F�rster-based fluorescence resonance energy transfer (FRET) probes and probes using non-FRET mechanisms. Energy transfer probes serve as molecule-size sensors, changing their fluorescence upon detection of various DNA reactions. 388 pp. Englisch. N� de r�f. du vendeur 9781617375316

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Fluorescent Energy Transfer Nucleic Acid Probes

Didenko, Vladimir V.

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Etat : New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Includes supplementary material: sn.pub/extrasFluorescent nucleic acid probes, which use energy transfer, include such constructs as molecular beacons, molecular break lights, Scorpion primers, TaqMan probes, and others. These probes signal detec. N� de r�f. du vendeur 4256881

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Fluorescent Energy Transfer Nucleic Acid Probes : Designs and Protocols

Vladimir V. Didenko

Edit� par Humana, 2010

ISBN 10 : 1617375314 ISBN 13 : 9781617375316

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Taschenbuch. Etat : Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - Fluorescent nucleic acid probes, which use energy transfer, include such constructs as molecular beacons, molecular break lights, Scorpion primers, TaqMan probes, and others. These probes signal detection of their targets by changing either the intensity or the color of their fluorescence. Not surpr- ingly, these luminous, multicolored probes carry more flashy names than their counterparts in the other fields of molecular biology. In recent years, fluor- cent probes and assays, which make use of energy transfer, have multiplied at a high rate and have found numerous applications. However, in spite of this explosive growth in the field, there are no manuals summarizing different p- tocols and fluorescent probe designs. In view of this, the main objective of Fluorescent Energy Transfer Nucleic Acid Probes: Designs and Protocols is to provide such a collection. Oligonucleotides with one or several chromophore tags can form fluor- cent probes capable of energy transfer. Energy transport within the probe can occur via the resonance energy transfer mechanism, also called F�rster tra- fer, or by non-F�rster transfer mechanisms. Although the probes using F�rster transfer were developed and used first, the later non-F�rster-based probes, such as molecular beacons, now represent an attractive and widely used option. The term 'fluorescent energy transfer probes' in the title of this book covers both F�rster-based fluorescence resonance energy transfer (FRET) probes and probes using non-FRET mechanisms. Energy transfer probes serve as molecule-size sensors, changing their fluorescence upon detection of various DNA reactions. N� de r�f. du vendeur 9781617375316

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Fluorescent Energy Transfer Nucleic Acid Probes

Vladimir V. Didenko

Edit� par Humana Dez 2010, 2010

ISBN 10 : 1617375314 ISBN 13 : 9781617375316

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Taschenbuch. Etat : Neu. This item is printed on demand - Print on Demand Titel. Neuware -Fluorescent nucleic acid probes, which use energy transfer, include such constructs as molecular beacons, molecular break lights, Scorpion primers, TaqMan probes, and others. These probes signal detection of their targets by changing either the intensity or the color of their fluorescence. Not surpr- ingly, these luminous, multicolored probes carry more flashy names than their counterparts in the other fields of molecular biology. In recent years, fluor- cent probes and assays, which make use of energy transfer, have multiplied at a high rate and have found numerous applications. However, in spite of this explosive growth in the field, there are no manuals summarizing different p- tocols and fluorescent probe designs. In view of this, the main objective of Fluorescent Energy Transfer Nucleic Acid Probes: Designs and Protocols is to provide such a collection. Oligonucleotides with one or several chromophore tags can form fluor- cent probes capable of energy transfer. Energy transport within the probe can occur via the resonance energy transfer mechanism, also called F�rster tra- fer, or by non-F�rster transfer mechanisms. Although the probes using F�rster transfer were developed and used first, the later non-F�rster-based probes, such as molecular beacons, now represent an attractive and widely used option. The term �fluorescent energy transfer probes� in the title of this book covers both F�rster-based fluorescence resonance energy transfer (FRET) probes and probes using non-FRET mechanisms. Energy transfer probes serve as molecule-size sensors, changing their fluorescence upon detection of various DNA reactions.Springer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 388 pp. Englisch. N� de r�f. du vendeur 9781617375316

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