RNA Silencing: Methods and Protocols - Couverture souple

Couverture souple

ISBN 10 : 1617375683 ISBN 13 : 9781617375682

Editeur : Humana, 2010

Synopsis

A collection of readily reproducible methods for the design, preparation, and use of RNAs for silencing gene expression in cells and organisms. The techniques range widely and include methods addressing the biochemical aspects of the silencing machinery, RNA silencing in non-mammalian organisms, and the in vivo delivery of siRNAs and silencing vectors. There are also techniques for designing, preparing, and using RNAs to silence gene expression, for fine-tuning regulation by targeting specific isoforms of a given gene, and for the study and use of microRNAs. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Détails de l'édition

• Éditeur: Humana
• Date d'édition: 2010
• Langue: anglais
• ISBN 10: 1617375683
• ISBN 13: 9781617375682
• Reliure: Broché
• Numéro d'édition: 1
• Nombre de pages: 352
• Éditeur: Carmichael Gordon
• Coordonnées du fabricant: non disponible
• Personne responsable: non disponible